Vol 49, No 2 (2011)
Original paper
Submitted: 2011-12-19
Published online: 2011-07-11
CD4+CD25+ and CD4+CD25high regulatory T cells in disseminated and localized forms of allergic contact dermatitis: relation to specific cytokines
Teresa Reduta, Anna Stasiak-Barmuta, Halina Laudańska
DOI: 10.5603/FHC.2011.0035
·
Folia Histochem Cytobiol 2011;49(2):255-262.
Vol 49, No 2 (2011)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-07-11
Abstract
The aim of this study was to evaluate regulatory T lymphocytes (Tregs) in the course of allergic
contact dermatitis (ACD) and to elucidate the role of IL-10 and TGF-b in Tregs activity. Peripheral blood
CD4+CD25+ and CD4+CD25high cells were determined by flow cytometry in patients with acute disseminated
ACD (‘ad’, n = 36), acute localized ACD (‘al’, n = 26), and disseminated ACD during remission (‘rd’, n = 27)
as well as in controls (n = 22). Serum levels of cytokines were measured using ELISA. The mean percentage of
CD4+CD25+ and CD4+CD25high cells in patients with ad ACD was significantly higher than in controls
(p < 0.01) and the remaining patients (p < 0.05). Both cell populations were significantly elevated in persons
with widespread skin lesions (p < 0.05). In ad patients the CD4+CD25+ increased during three weeks of
disease, although the significant increase of CD4+CD25high was noted only in the third week. Patients with ad
ACD showed a significantly decreased serum level of TGF-b1 as compared with controls and the remaining
ACD patients. IL-10 level did not differ between all groups. The elevated population of CD4+CD25high cells in
ad ACD patients, and its dependence on the extension of skin lesions, suggest a role of Tregs in regulating the
course of ACD. The growing Tregs percentages may indicate their peripheral generation during ACD. The
development of lesions despite an increased population of Tregs suggests their functional defect. The role of
TGF-b1 in the suppressive activity of Tregs cannot be excluded. (Folia Histochemica et Cytobiologica 2011;
Vol. 49, No. 2, pp. 255–262)
Abstract
The aim of this study was to evaluate regulatory T lymphocytes (Tregs) in the course of allergic
contact dermatitis (ACD) and to elucidate the role of IL-10 and TGF-b in Tregs activity. Peripheral blood
CD4+CD25+ and CD4+CD25high cells were determined by flow cytometry in patients with acute disseminated
ACD (‘ad’, n = 36), acute localized ACD (‘al’, n = 26), and disseminated ACD during remission (‘rd’, n = 27)
as well as in controls (n = 22). Serum levels of cytokines were measured using ELISA. The mean percentage of
CD4+CD25+ and CD4+CD25high cells in patients with ad ACD was significantly higher than in controls
(p < 0.01) and the remaining patients (p < 0.05). Both cell populations were significantly elevated in persons
with widespread skin lesions (p < 0.05). In ad patients the CD4+CD25+ increased during three weeks of
disease, although the significant increase of CD4+CD25high was noted only in the third week. Patients with ad
ACD showed a significantly decreased serum level of TGF-b1 as compared with controls and the remaining
ACD patients. IL-10 level did not differ between all groups. The elevated population of CD4+CD25high cells in
ad ACD patients, and its dependence on the extension of skin lesions, suggest a role of Tregs in regulating the
course of ACD. The growing Tregs percentages may indicate their peripheral generation during ACD. The
development of lesions despite an increased population of Tregs suggests their functional defect. The role of
TGF-b1 in the suppressive activity of Tregs cannot be excluded. (Folia Histochemica et Cytobiologica 2011;
Vol. 49, No. 2, pp. 255–262)
Keywords
allergic contact dermatitis; clinical course; regulation; regulatory T lymphocytes
Title
CD4+CD25+ and CD4+CD25high regulatory T cells in disseminated and localized forms of allergic contact dermatitis: relation to specific cytokines
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 2 (2011)
Article type
Original paper
Pages
255-262
Published online
2011-07-11
Page views
1605
Article views/downloads
7601
DOI
10.5603/FHC.2011.0035
Bibliographic record
Folia Histochem Cytobiol 2011;49(2):255-262.
Keywords
allergic contact dermatitis
clinical course
regulation
regulatory T lymphocytes
Authors
Teresa Reduta
Anna Stasiak-Barmuta
Halina Laudańska
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