open access

Vol 49, No 3 (2011)
ORIGINAL PAPERS
Published online: 2011-10-28
Submitted: 2012-01-05
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Different distribution of c-kit positive interstitial cells of Cajal-like in children’s urinary bladders

Anna Małgorzata Piaseczna-Piotrowska, Monika Dzieniecka, Andrzej Kulig, Marian Danilewicz, Andrzej Chilarski
DOI: 10.5603/FHC.2011.0061
·
Folia Histochem Cytobiol 2011;49(3):431-435.

open access

Vol 49, No 3 (2011)
ORIGINAL PAPERS
Published online: 2011-10-28
Submitted: 2012-01-05

Abstract

We describe the presence of c-kit positive interstitial cells of Cajal-like (ICCs-like) in the walls of the urinary bladders of children. An immunohistochemical study of specimens, obtained at autopsy from either the trigonum (Group A) or the corpus (Group B), was performed using antibodies against c-kit (CD 117). Histological morphometry of the immunoexpression of c-kit positive ICCs-like was performed by means of image analysis system. The c-kit positive ICCs-like were identified by their morphology and counted in the vesical muscle layer in ten adjacent high power fields, each of 0.0479 mm2. The areas of the epithelial and subepithelial layers containing c-kit positive mast cells (rounded body with no dendritic processes) were neglected. The results were expressed as the number of ICCs-like cells per mm2. Differences between groups were tested using unpaired Student’s t-test preceded by evaluation of normality and Levene’s test. Results were considered statistically significant if p < 0.05. In Group A, the mean number of ICCs-like cells was statistically significantly higher (41.5 cells/mm2) than in Group B (30.4 cells/mm2), p < 0.05. ICCs-like cells were found within the smooth muscle layer of the urinary bladder. There was a different distribution of these cells in particular parts of the bladder, which was probably due to the different roles of the trigonum and the corpus in the bladders of children. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 431–435)

Abstract

We describe the presence of c-kit positive interstitial cells of Cajal-like (ICCs-like) in the walls of the urinary bladders of children. An immunohistochemical study of specimens, obtained at autopsy from either the trigonum (Group A) or the corpus (Group B), was performed using antibodies against c-kit (CD 117). Histological morphometry of the immunoexpression of c-kit positive ICCs-like was performed by means of image analysis system. The c-kit positive ICCs-like were identified by their morphology and counted in the vesical muscle layer in ten adjacent high power fields, each of 0.0479 mm2. The areas of the epithelial and subepithelial layers containing c-kit positive mast cells (rounded body with no dendritic processes) were neglected. The results were expressed as the number of ICCs-like cells per mm2. Differences between groups were tested using unpaired Student’s t-test preceded by evaluation of normality and Levene’s test. Results were considered statistically significant if p < 0.05. In Group A, the mean number of ICCs-like cells was statistically significantly higher (41.5 cells/mm2) than in Group B (30.4 cells/mm2), p < 0.05. ICCs-like cells were found within the smooth muscle layer of the urinary bladder. There was a different distribution of these cells in particular parts of the bladder, which was probably due to the different roles of the trigonum and the corpus in the bladders of children. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 431–435)
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Keywords

interstitial cells of Cajal; CD 117; immunohistochemistry; urinary bladder

About this article
Title

Different distribution of c-kit positive interstitial cells of Cajal-like in children’s urinary bladders

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 3 (2011)

Pages

431-435

Published online

2011-10-28

DOI

10.5603/FHC.2011.0061

Bibliographic record

Folia Histochem Cytobiol 2011;49(3):431-435.

Keywords

interstitial cells of Cajal
CD 117
immunohistochemistry
urinary bladder

Authors

Anna Małgorzata Piaseczna-Piotrowska
Monika Dzieniecka
Andrzej Kulig
Marian Danilewicz
Andrzej Chilarski

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