open access

Vol 49, No 3 (2011)
Original paper
Submitted: 2012-01-05
Published online: 2011-10-28
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Comparing the content of lipids derived from the eye lenses of various species

Tomasz Panz, Magdalena Lepiarczyk, Agnieszka Żuber
DOI: 10.5603/FHC.2011.0060
·
Folia Histochem Cytobiol 2011;49(3):425-430.

open access

Vol 49, No 3 (2011)
ORIGINAL PAPERS
Submitted: 2012-01-05
Published online: 2011-10-28

Abstract

The lipid content in the eye lens was analyzed and compared among various species in this study. The eye lens lipids of the following species were investigated: cow, horse, duck, and freshwater trout. Additionally, the lipids derived from cataractous bovine lens and from cataractous human eye lens lipoprotein complexes were analyzed. The following lipid classes were detected in clear lenses: cholesterol, sphingomyelin, phosphatidylcholine, phosphatidyletanolamine, and phosphatidylserine. In cataractous bovine lens and in lipoprotein complexes from human nuclear cataract, phosphatidyloinositol and phosphatidyloglycerol were detected. Cholesterol and sphingomyelin, essential for hypothetical formation of cholesterol-rich domains, were the most abundant lipids in the lenses of all investigated species. These two components of eye lens lipid fraction were analyzed quantitatively using thin layer chromatography and spectrophotometric assay; the other lipids were identified qualitatively using thin layer chromatography. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 425–430)

Abstract

The lipid content in the eye lens was analyzed and compared among various species in this study. The eye lens lipids of the following species were investigated: cow, horse, duck, and freshwater trout. Additionally, the lipids derived from cataractous bovine lens and from cataractous human eye lens lipoprotein complexes were analyzed. The following lipid classes were detected in clear lenses: cholesterol, sphingomyelin, phosphatidylcholine, phosphatidyletanolamine, and phosphatidylserine. In cataractous bovine lens and in lipoprotein complexes from human nuclear cataract, phosphatidyloinositol and phosphatidyloglycerol were detected. Cholesterol and sphingomyelin, essential for hypothetical formation of cholesterol-rich domains, were the most abundant lipids in the lenses of all investigated species. These two components of eye lens lipid fraction were analyzed quantitatively using thin layer chromatography and spectrophotometric assay; the other lipids were identified qualitatively using thin layer chromatography. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 425–430)
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Keywords

eye lens; cataract; cholesterol; sphingomyelin; thin layer chromatography

About this article
Title

Comparing the content of lipids derived from the eye lenses of various species

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 3 (2011)

Article type

Original paper

Pages

425-430

Published online

2011-10-28

DOI

10.5603/FHC.2011.0060

Bibliographic record

Folia Histochem Cytobiol 2011;49(3):425-430.

Keywords

eye lens
cataract
cholesterol
sphingomyelin
thin layer chromatography

Authors

Tomasz Panz
Magdalena Lepiarczyk
Agnieszka Żuber

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