open access

Vol 49, No 3 (2011)
ORIGINAL PAPERS
Published online: 2011-10-28
Submitted: 2012-01-05
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Expression of pattern recognition receptors in liver biopsy specimens of children chronically infected with HBV and HCV

Iwona Mozer-Lisewska, Arleta Kowala-Piaskowska, Anna Mania, Renata Jenek, Husam Samara, Elżbieta Kaczmarek, Jan Sikora, Wojciech Służewski, Jan Żeromski
DOI: 10.5603/FHC.2011.0058
·
Folia Histochem Cytobiol 2011;49(3):410-416.

open access

Vol 49, No 3 (2011)
ORIGINAL PAPERS
Published online: 2011-10-28
Submitted: 2012-01-05

Abstract

Pattern recognition receptors (PRRs) constitute a pivotal arm of innate immunity. Their distribution is widespread and not limited to cells of the immune system. Following our previous findings concerning the expression of Toll-like receptors (TLRs) 2, 3 and 4 in chronic viral hepatitis C of children, we wished to search for other PRRs, including other TLRs, NOD-like receptors (NLRs) and RIG-1-like helicase receptors (RLR) in infected hepatocytes. Liver biopsy fragments from ten children with chronic hepatitis B and C were used and two others in which hepatotropic virus infection was excluded. Frozen sections of liver samples were subjected to ABC immunohistochemistry (IHC) following incubation with a set of antibodies. Results of IHC findings were screened for correlation with clinical/laboratory data of patients. It was found that several PRRs could be shown in affected hepatocytes, but the incidence was higher in hepatitis C than in B. In hepatitis C, TLR1, 2, 4, NALP and RIG-1 helicase showed the most marked expression. In hepatitis B, TLR1, 3, 9, NOD1 and NALP expression were the most conspicuous. Expression PRRs in liver from hepatitis of unknown origin was much lower. It was also the case in cytospins from human hepatoma cell line. Several correlations between PRRs expression and clinical findings in patients could be shown by statistical exploration. In conclusion, this data suggests some role for PRRs in the pathogenesis of chronic viral hepatitis. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 410–416)

Abstract

Pattern recognition receptors (PRRs) constitute a pivotal arm of innate immunity. Their distribution is widespread and not limited to cells of the immune system. Following our previous findings concerning the expression of Toll-like receptors (TLRs) 2, 3 and 4 in chronic viral hepatitis C of children, we wished to search for other PRRs, including other TLRs, NOD-like receptors (NLRs) and RIG-1-like helicase receptors (RLR) in infected hepatocytes. Liver biopsy fragments from ten children with chronic hepatitis B and C were used and two others in which hepatotropic virus infection was excluded. Frozen sections of liver samples were subjected to ABC immunohistochemistry (IHC) following incubation with a set of antibodies. Results of IHC findings were screened for correlation with clinical/laboratory data of patients. It was found that several PRRs could be shown in affected hepatocytes, but the incidence was higher in hepatitis C than in B. In hepatitis C, TLR1, 2, 4, NALP and RIG-1 helicase showed the most marked expression. In hepatitis B, TLR1, 3, 9, NOD1 and NALP expression were the most conspicuous. Expression PRRs in liver from hepatitis of unknown origin was much lower. It was also the case in cytospins from human hepatoma cell line. Several correlations between PRRs expression and clinical findings in patients could be shown by statistical exploration. In conclusion, this data suggests some role for PRRs in the pathogenesis of chronic viral hepatitis. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 410–416)
Get Citation

Keywords

pattern recognition receptors; viral hepatitis; liver biopsy; children; immunohistochemistry

About this article
Title

Expression of pattern recognition receptors in liver biopsy specimens of children chronically infected with HBV and HCV

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 3 (2011)

Pages

410-416

Published online

2011-10-28

DOI

10.5603/FHC.2011.0058

Bibliographic record

Folia Histochem Cytobiol 2011;49(3):410-416.

Keywords

pattern recognition receptors
viral hepatitis
liver biopsy
children
immunohistochemistry

Authors

Iwona Mozer-Lisewska
Arleta Kowala-Piaskowska
Anna Mania
Renata Jenek
Husam Samara
Elżbieta Kaczmarek
Jan Sikora
Wojciech Służewski
Jan Żeromski

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