Vol 60, No 2 (2022)
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Published online: 2022-06-06

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Distribution of neuropeptide Y in the brain of the male native Thai chicken

Boonyarit Kamkrathok1, Yupaporn Chaiseha2
Pubmed: 35661992
Folia Histochem Cytobiol 2022;60(2):146-155.


Introduction. Neuropeptide Y (NPY), a 36 amino acid neurotransmitter/neuromodulator, is involved in food intake and parental cares in birds. NPY is associated with the regulation of the reproductive system in the female native Thai chickens. However, the role of NPY in the male native Thai chicken has not been studied. Therefore, the objective of this study was to investigate the distributions of NPY immunoreactive (-ir) neurons and fibers in the brain of the male native Thai chickens.
Material and methods. The distribution of NPY-ir neurons and fibers in the hen brain was elucidated utilizing immunohistochemical technique.
Results. The distributions of NPY-ir neurons and fibers were located throughout the brain, predominantly in the hypothalamus. The numbers of NPY-ir neurons within the nucleus paraventricularis magnocellularis (PVN) were significantly higher than those of the nucleus septalis lateralis (SL), nucleus supraopticus (SOv), and nucleus inferioris hypothalami and nucleus infundibuli hypothalami (IH-IN). In addition, the numbers of NPY-ir neurons within the SL, SOv, and IH-IN were significantly higher than those of the tractus septomesencephalicus and nucleus dorsolateralis anterior thalami.
Conclusions. These results indicated, for the first time, that the distributions of NPY-ir neurons and fibers in the brain of the male native Thai chickens were markedly observed in the hypothalamus, especially within the PVN, implicating that the NPYergic system within the PVN might be related to the regulation of feeding behavior and parental cares in this equatorial species.

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  1. Tatemoto K. Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci U S A. 1982; 79(18): 5485–5489.
  2. Tatemoto K, Carlquist M, Mutt V. Neuropeptide Y - a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature. 1982; 296(5858): 659–660.
  3. Blomqvist AG, Söderberg C, Lundell I, et al. Strong evolutionary conservation of neuropeptide Y: sequences of chicken, goldfish, and Torpedo marmorata DNA clones. Proc Natl Acad Sci U S A. 1992; 89(6): 2350–2354.
  4. Larhammar D. Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide. Regulatory Peptides. 1996; 62(1): 1–11.
  5. Kuenzel WJ, Douglass LW, Davison BA. Robust feeding following central administration of neuropeptide Y or peptide YY in chicks, Gallus domesticus. Peptides. 1987; 8(5): 823–828.
  6. Richardson RD, Boswell T, Raffety BD, et al. NPY increases food intake in white-crowned sparrows: effect in short and long photoperiods. Am J Physiol. 1995; 268(6 Pt 2): R1418–R1422.
  7. Yi J, Gilbert E, Siegel P, et al. Fed and fasted chicks from lines divergently selected for low or high body weight have differential hypothalamic appetite-associated factor mRNA expression profiles. Behavioural Brain Research. 2015; 286: 58–63.
  8. Fraley GS, Kuenzel WJ. Precocious puberty in chicks (Gallus domesticus) induced by central injections of neuropeptide Y. Life Sci. 1993; 52(20): 1649–1656.
  9. Walsh K, Kuenzel W. Effect of Sulfamethazine on Sexual Precocity and Neuropeptide Y Neurons Within the Tuberoinfundibular Region of the Chick Brain. Brain Research Bulletin. 1997; 44(6): 707–713.
  10. Harding RL, Clark DL, Halevy O, et al. The effect of temperature on apoptosis and adipogenesis on skeletal muscle satellite cells derived from different muscle types. Physiol Rep. 2015; 3(9).
  11. Clark DL, McCormick JL, Velleman SG. Effect of incubation temperature on neuropeptide Y and neuropeptide Y receptors in turkey and chicken satellite cells. Comp Biochem Physiol A Mol Integr Physiol. 2018; 219-220: 58–66.
  12. Chowdhury VS. Heat stress biomarker amino acids and neuropeptide afford thermotolerance in chicks. J Poult Sci. 2019; 56(1): 1–11.
  13. Kuenzel WJ, McMurtry J. Neuropeptide Y: brain localization and central effects on plasma insulin levels in chicks. Physiol Behav. 1988; 44(4-5): 669–678.
  14. Contijoch AM, Malamed S, McDonald JK, et al. Neuropeptide Y regulation of LHRH release in the median eminence: immunocytochemical and physiological evidence in hens. Neuroendocrinology. 1993; 57(1): 135–145.
  15. Barker-Gibb ML, Scott CJ, Boublik JH, et al. The role of neuropeptide Y (NPY) in the control of LH secretion in the ewe with respect to season, NPY receptor subtype and the site of action in the hypothalamus. J Endocrinol. 1995; 147(3): 565–579.
  16. Ramakrishnan S, Strader AD, Wimpee B, et al. Evidence for increased neuropeptide Y synthesis in mediobasal hypothalamus in relation to parental hyperphagia and gonadal activation in breeding ring doves. J Neuroendocrinol. 2007; 19(3): 163–171.
  17. Davies S, Cros T, Richard D, et al. Food availability, energetic constraints and reproductive development in a wild seasonally breeding songbird. Funct Ecol. 2015; 29(11): 1421–1434.
  18. Valle S, Carpentier E, Vu B, et al. Food restriction negatively affects multiple levels of the reproductive axis in male house finches, Haemorhous mexicanus. J Exp Biol. 2015; 218(Pt 17): 2694–2704.
  19. Furuse M, Matsumoto M, Mori R, et al. Influence of fasting and neuropeptide Y on the suppressive food intake induced by intracerebroventricular injection of glucagon-like peptide-1 in the neonatal chick. Brain Res. 1997; 764(1-2): 289–292.
  20. Ando R, Kawakami SI, Bungo T, et al. Feeding responses to several neuropeptide Y receptor agonists in the neonatal chick. Eur J Pharmacol. 2001; 427(1): 53–59.
  21. Cline MA, Smith ML. Central alpha-melanocyte stimulating hormone attenuates behavioral effects of neuropeptide Y in chicks. Physiol Behav. 2007; 91(5): 588–592.
  22. McConn BR, Gilbert ER, Cline MA. Appetite-associated responses to central neuropeptide Y injection in quail. Neuropeptides. 2018; 69: 9–18.
  23. Boswell T, Dunn IC, Corr SA. Hypothalamic neuropeptide Y mRNA is increased after feed restriction in growing broilers. Poult Sci. 1999; 78(8): 1203–1207.
  24. Boswell T, Li Q, Takeuchi S. Neurons expressing neuropeptide Y mRNA in the infundibular hypothalamus of Japanese quail are activated by fasting and co-express agouti-related protein mRNA. Brain Res Mol Brain Res. 2002; 100(1-2): 31–42.
  25. Zhou W, Murakami M, Hasegawa S, et al. Neuropeptide Y content in the hypothalamic paraventricular nucleus responds to fasting and refeeding in broiler chickens. Comp Biochem Physiol A Mol Integr Physiol. 2005; 141(2): 146–152.
  26. Lees JJ, Lindholm C, Batakis P, et al. The physiological and neuroendocrine correlates of hunger in the Red Junglefowl (Gallus gallus). Sci Rep. 2017; 7(1): 17984.
  27. McConn BR, Gilbert ER, Cline MA. Fasting and refeeding induce differential changes in hypothalamic mRNA abundance of appetite-associated factors in 7 day-old Japanese quail, Coturnix japonica. Comp Biochem Physiol A Mol Integr Physiol. 2019; 227: 60–67.
  28. Sartsoongnoen N, Kamkrathok B, Songserm T, et al. Distribution and variation of neuropeptide Y in the brain of native Thai chicken. Avian Biology Research. 2020; 14(1): 27–36.
  29. Kamkrathok B, Sartsoongnoen N, Chaiseha Y, et al. Role of vasoactive intestinal peptide during the transition from incubation behavior to rearing behavior in the female native Thai chicken. Poult Sci. 2017; 96(10): 3768–3774.
  30. Boswell T, Millam JR, Li Q, et al. Cellular localization of neuropeptide Y mRNA and peptide in the brain of the Japanese quail and domestic chicken. Cell Tissue Res. 1998; 293(1): 31–38.
  31. Esposito V, Pelagalli GV, De Girolamo P, et al. Anatomical distribution of NPY-like immunoreactivity in the domestic chick brain (Gallus domesticus). Anat Rec. 2001; 263(2): 186–201.
  32. Aste N, Viglietti-Panzica C, Fasolo A, et al. Localization of neuropeptide Y-immunoreactive cells and fibres in the brain of the Japanese quail. Cell Tissue Res. 1991; 265(2): 219–230.
  33. Anderson KD, Reiner A. Distribution and relative abundance of neurons in the pigeon forebrain containing somatostatin, neuropeptide Y, or both. J Comp Neurol. 1990; 299(3): 261–282.
  34. Lorenz E, Skofitsch G. Immunohistochemical localization of neuropeptide Y in the central nervous system of the dove (Columba livia). In: Elsner N, Roth G. ed. Brain-perception-cognition. Thieme, Stuttgart 1990: 326.
  35. Gould KL, Newman SW, Tricomi EM, et al. The distribution of substance P and neuropeptide Y in four songbird species: a comparison of food-storing and non-storing birds. Brain Res. 2001; 918(1-2): 80–95.
  36. den Boer-Visser AMd, Dubbeldam JL. The distribution of dopamine, substance P, vasoactive intestinal polypeptide and neuropeptide Y immunoreactivity in the brain of the collared dove, Streptopelia decaocto. Journal of Chemical Neuroanatomy. 2002; 23(1): 1–27.
  37. Radzimirska M, Bogus-Nowakowska K, Kuder T, et al. Distribution of cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) in the pterygopalatine ganglion of the domestic duck (Anas platyrhynchos f. domestica). Folia Histochem Cytobiol. 2016; 54(1): 25–31.
  38. Austic RE, Nesheim MC. Poultry Production (13th ed.). Lea & Febiger, Philadelphia 1990.
  39. Fumihito A, Miyake T, Sumi S, et al. One subspecies of the red junglefowl (Gallus gallus gallus) suffices as the matriarchic ancestor of all domestic breeds. Proc Natl Acad Sci USA. 1994; 91(26): 12505–12509.
  40. Hillel J, Groenen MAM, Tixier-Boichard M, et al. Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools. Genet Sel Evol. 2003; 35(5): 533–557.
  41. Sawai H, Kim HL, Kuno K, et al. The origin and genetic variation of domestic chickens with special reference to junglefowls Gallus g. gallus and G. varius. PLoS One. 2010; 5(5): e10639.
  42. Chaiseha Y, Halawani MEl. Brooding. Sturkie's Avian Physiology. 2015: 717–738.
  43. Namken S, Sinpru P, Kamkrathok B, et al. Role of vasoactive intestinal peptide during the transition from incubation behavior to rearing behavior in the female native Thai chicken. Poult Sci. 2017; 96(10): 3768–3774.
  44. Sinpru P, Porter TE, El Halawani ME, et al. Effects of nest-deprivation on hypothalamic mesotocin in incubating native Thai hens (Gallus domesticus). Acta Histochem. 2017; 119(7): 708–718.
  45. Sinpru P, Sartsoongnoen N, Rozenboim I, et al. The effects of replacing eggs with chicks on mesotocin, dopamine, and prolactin in the native Thai hen. Gen Comp Endocrinol. 2018; 263: 32–42.
  46. Kamkrathok B, Sartsoongnoen N, Prakobsaeng N, et al. Distribution of hypothalamic vasoactive intestinal peptide immunoreactive neurons in the male native Thai chicken. Anim Reprod Sci. 2016; 171: 27–35.
  47. Kamkrathok B, Porter TE, El Halawani ME, et al. Distribution of mesotocin-immunoreactive neurons in the brain of the male native Thai chicken. Acta Histochem. 2017; 119(8): 804–811.
  48. Kamkrathok B, Chaiseha Y. Distribution of dopamine-immunoreactive neurons in the brain of the male native Thai chicken. Folia Histochem Cytobiol. 2022; 60(1): 1–12.
  49. Lynn SE, Perfito N, Guardado D, et al. Food, stress, and circulating testosterone: Cue integration by the testes, not the brain, in male zebra finches (Taeniopygia guttata). Gen Comp Endocrinol. 2015; 215: 1–9.
  50. Yee CL, Wang Y, Anderson S, et al. Arcuate nucleus expression of NKX2.1 and DLX and lineages expressing these transcription factors in neuropeptide Y(+), proopiomelanocortin(+), and tyrosine hydroxylase(+) neurons in neonatal and adult mice. J Comp Neurol. 2009; 517(1): 37–50.
  51. Ibáñez-Sandoval O, Tecuapetla F, Unal B, et al. A novel functionally distinct subtype of striatal neuropeptide Y interneuron. J Neurosci. 2011; 31(46): 16757–16769.
  52. Lee SJ, Kirigiti M, Lindsley SR, et al. Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models. J Comp Neurol. 2013; 521(8): 1891–1914.
  53. Chokchaloemwong D, Prakobsaeng N, Sartsoongnoen N, et al. Mesotocin and maternal care of chicks in native Thai hens (Gallus domesticus). Horm Behav. 2013; 64(1): 53–69.
  54. Kuenzel WJ, van Tienhoven A. Nomenclature and location of avian hypothalamic nuclei and associated circumventricular organs. J Comp Neurol. 1982; 206(3): 293–313.
  55. Wang X, Day JR, Vasilatos-Younken R. The distribution of neuropeptide Y gene expression in the chicken brain. Mol Cell Endocrinol. 2001; 174(1-2): 129–136.
  56. Kuenzel W, Beck M, Teruyama R. Neural sites and pathways regulating food intake in birds: A comparative analysis to mammalian systems. J Exp Zool. 1999; 283(4-5): 348–364, doi: 10.1002/(sici)1097-010x(19990301/01)283:4/5<348::aid-jez5>3.0.co;2-5.
  57. Chen GQ, Hu XF, Sugahara K, et al. Type-dependent differential expression of neuropeptide Y in chicken hypothalamus (Gallus domesticus). J Zhejiang Univ Sci B. 2007; 8(11): 839–844.
  58. Youngren O, Chaiseha Y, Phillips R, et al. Vasoactive intestinal peptide concentrations in turkey hypophysial portal blood differ across the reproductive cycle. Gen Comp Endocrinol. 1996; 103(3): 323–330.
  59. Mauro LJ, Elde RP, Youngren OM, et al. Alterations in hypothalamic vasoactive intestinal peptide-like immunoreactivity are associated with reproduction and prolactin release in the female turkey. Endocrinology. 1989; 125(4): 1795–1804.
  60. Chaiseha Y, El Halawani ME. Expression of vasoactive intestinal peptide/peptide histidine isoleucine in several hypothalamic areas during the turkey reproductive cycle: relationship to prolactin secretion. Neuroendocrinology. 1999; 70(6): 402–412.
  61. Chaiseha Y, Tong Z, Youngren OM, et al. Transcriptional changes in hypothalamic vasoactive intestinal peptide during a photo-induced reproductive cycle in the turkey. J Mol Endocrinol. 1998; 21(3): 267–275.
  62. Contijoch AM, Gonzalez C, Singh HN, et al. Dopaminergic regulation of luteinizing hormone-releasing hormone release at the median eminence level: immunocytochemical and physiological evidence in hens. Neuroendocrinology. 1992; 55(3): 290–300.
  63. Fraley GS, Kuenzel WJ. Immunocytochemical and histochemical analyses of gonadotrophin releasing hormone, tyrosine hydroxylase, and cytochrome oxidase reactivity within the hypothalamus of chicks showing early sexual maturation. Histochemistry. 1993; 99(3): 221–229.
  64. Advis JP, Contijoch AM. The median eminence as a site for neuroendocrine control of reproduction in hens. Poult Sci. 1993; 72(5): 932–939.
  65. Kalra SP, Dube MG, Pu S, et al. Interacting appetite-regulating pathways in the hypothalamic regulation of body weight. Endocr Rev. 1999; 20(1): 68–100.
  66. Strader AD, Buntin JD. Neuropeptide-Y: a possible mediator of prolactin-induced feeding and regulator of energy balance in the ring dove (Streptopelia risoria). J Neuroendocrinol. 2001; 13(4): 386–392.