open access

Vol 59, No 3 (2021)
Original paper
Submitted: 2021-05-18
Accepted: 2021-09-24
Published online: 2021-09-27
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SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the JAK2/STAT3 pathway

Wen Xiong1, Xin Guo1, Xianhua Cai1
DOI: 10.5603/FHC.a2021.0020
·
Pubmed: 34580847
·
Folia Histochem Cytobiol 2021;59(3):187-194.
Affiliations
  1. The First School of Clinical Medicine, Southern Medical University, Guangdong, China

open access

Vol 59, No 3 (2021)
ORIGINAL PAPERS
Submitted: 2021-05-18
Accepted: 2021-09-24
Published online: 2021-09-27

Abstract

Introduction. This study aimed to investigate the effects of stromal cell-derived factor-1 (SDF-1) and activation of its receptor, chemokine receptor 4 (CXCR4), on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and the key signaling mechanisms involved in these effects.

Material and methods. BMSCs were treated with 100 μg/L SDF-1 and cultured in osteogenic medium for 7 days. RT-qPCR and western blotting were used to detect the protein and mRNA levels of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), Runt-related transcription factor 2 (Runx2), and osteocalcin (OCN). Alizarin-red staining was used to detect the mineralization-inducing ability of the cells.

Results. After BMSCs were treated with SDF-1, the levels of JAK2 mRNA, STAT3 mRNA, and protein phosphorylation increased, the number of mineralized nodules of BMSCs increased, and the osteogenic-differentiation ability was enhanced. In addition, after BMSCs were treated with an inhibitor of JAK2 phosphorylation, the levels of JAK2, STAT3, Runx2, and OCN decreased significantly, the number of mineralized nodules of BMSCs also decreased, and the osteogenic-differentiation ability decreased. The inhibition of CXCR4-treated BMSCs further confirmed that SDF-1/CXCR4 activated JAK2/STAT3 to regulate the osteogenic differentiation of BMSCs.

Conclusions. SDF-1/CXCR4 promoted the osteogenic differentiation of BMSCs through JAK2/STAT3 activation.

Abstract

Introduction. This study aimed to investigate the effects of stromal cell-derived factor-1 (SDF-1) and activation of its receptor, chemokine receptor 4 (CXCR4), on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and the key signaling mechanisms involved in these effects.

Material and methods. BMSCs were treated with 100 μg/L SDF-1 and cultured in osteogenic medium for 7 days. RT-qPCR and western blotting were used to detect the protein and mRNA levels of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), Runt-related transcription factor 2 (Runx2), and osteocalcin (OCN). Alizarin-red staining was used to detect the mineralization-inducing ability of the cells.

Results. After BMSCs were treated with SDF-1, the levels of JAK2 mRNA, STAT3 mRNA, and protein phosphorylation increased, the number of mineralized nodules of BMSCs increased, and the osteogenic-differentiation ability was enhanced. In addition, after BMSCs were treated with an inhibitor of JAK2 phosphorylation, the levels of JAK2, STAT3, Runx2, and OCN decreased significantly, the number of mineralized nodules of BMSCs also decreased, and the osteogenic-differentiation ability decreased. The inhibition of CXCR4-treated BMSCs further confirmed that SDF-1/CXCR4 activated JAK2/STAT3 to regulate the osteogenic differentiation of BMSCs.

Conclusions. SDF-1/CXCR4 promoted the osteogenic differentiation of BMSCs through JAK2/STAT3 activation.

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Keywords

BMSCs; osteogenic differentiation; SDF-1; CXCR4; JAK2/STAT3; Runx2, osteocalcin; alizarin red

About this article
Title

SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the JAK2/STAT3 pathway

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 59, No 3 (2021)

Article type

Original paper

Pages

187-194

Published online

2021-09-27

DOI

10.5603/FHC.a2021.0020

Pubmed

34580847

Bibliographic record

Folia Histochem Cytobiol 2021;59(3):187-194.

Keywords

BMSCs
osteogenic differentiation
SDF-1
CXCR4
JAK2/STAT3
Runx2
osteocalcin
alizarin red

Authors

Wen Xiong
Xin Guo
Xianhua Cai

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