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Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells
Affiliations- Institute of Reproduction Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China
- Department of Physiology, Medical School of Jianghan University, Wuhan, China
- Department of Human Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
open access
Abstract
Introduction. Urokinase plasminogen activator (uPA) is a serine protease and it also demonstrates proinflammatory properties. We thus hypothesized that uPA is involved in immunity of Sertoli cells in the rat testis.
Materials and methods. The uPA gene(PLAU) promoter was cloned by RT-PCR and the transcriptional activation of core domain was screened by using Dual-Luciferase Reporter Assay System. The Sertoli cells were harvested from 20-day-old Sprague-Dawley male rats and total RNA was isolated. The uPA mRNA levels and MyD88 pathway were tested by qPCR.
Results. We successfully cloned the 1517-bp rat uPA gene and screened the core domain (-455/+40) in five
different fragments of uPA promoter. The uPA expression and uPA promoter activity were upregulated in
lipopolysaccharide (LPS)-stimulated Sertoli cells. Furthermore, the uPA expression was regulated through the
MyD88-independent pathway by interdicting IRF3 and interferon b.
Conclusion. uPA expression is likely induced by LPS through the core promoter domain of uPA. This finding
implied that uPA played a role in the immune function of Sertoli cells in rat testis, which might provide the development of new treatments for male infertility.
Abstract
Introduction. Urokinase plasminogen activator (uPA) is a serine protease and it also demonstrates proinflammatory properties. We thus hypothesized that uPA is involved in immunity of Sertoli cells in the rat testis.
Materials and methods. The uPA gene(PLAU) promoter was cloned by RT-PCR and the transcriptional activation of core domain was screened by using Dual-Luciferase Reporter Assay System. The Sertoli cells were harvested from 20-day-old Sprague-Dawley male rats and total RNA was isolated. The uPA mRNA levels and MyD88 pathway were tested by qPCR.
Results. We successfully cloned the 1517-bp rat uPA gene and screened the core domain (-455/+40) in five
different fragments of uPA promoter. The uPA expression and uPA promoter activity were upregulated in
lipopolysaccharide (LPS)-stimulated Sertoli cells. Furthermore, the uPA expression was regulated through the
MyD88-independent pathway by interdicting IRF3 and interferon b.
Conclusion. uPA expression is likely induced by LPS through the core promoter domain of uPA. This finding
implied that uPA played a role in the immune function of Sertoli cells in rat testis, which might provide the development of new treatments for male infertility.
Keywords
rat; Sertoli cells; urokinase plasminogen activator; lipopolysaccharide; uPA promoter
About this articleTitle
Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
236-244
Published online
2021-11-30
Page views
6764
Article views/downloads
468
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2021;59(4):236-244.
Keywords
rat
Sertoli cells
urokinase plasminogen activator
lipopolysaccharide
uPA promoter
Authors
Yan Liu
Kai Zhao
Zhe Xiong
Yuanxiao Yi
Chengliang Xiong
Donghui Huang
Hu Zhao
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