open access

Vol 59, No 1 (2021)
Original paper
Submitted: 2020-09-21
Accepted: 2021-01-28
Published online: 2021-02-09
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miR-140-5p inhibits the proliferation, migration and invasion of vascular smooth muscle cells by suppressing the expression of NCKAP1

Qing Ma1, Jiancheng Liu1, Chunbo Li1, Dong Wang1
DOI: 10.5603/FHC.a2021.0003
·
Pubmed: 33560516
·
Folia Histochem Cytobiol 2021;59(1):22-29.
Affiliations
  1. Cardiovascular Surgery, Tangshan Gongren Hospital, Tangshan, Hebei Province, 063000, China

open access

Vol 59, No 1 (2021)
ORIGINAL PAPERS
Submitted: 2020-09-21
Accepted: 2021-01-28
Published online: 2021-02-09

Abstract

Introduction. The occurrence of aortic dissection is related to the proliferation and metastasis of vascular smooth muscle cells. In our present study, we found that the expression of miR-140-5p was inhibited in the wall of abdominal aorta of aortic dissection patients. However, the mechanism of miR-140-5p in the development of aortic dissection is unclear.

Material and methods. We detected the expression of miR-140-5p and NCK Associated Protein 1 (NCKAP1) in blood vessel of aortic dissection patients and normal people by PCR. Next, we established the miR-140-5p overexpression and miR-140-5p inhibition vascular smooth muscle cells (CRL-1999 cells). The BrdU assays, wound healing assays and transwell assays were performed to detect the proliferation and invasion ability of these cells. Finally, luciferase reporter assay was performed to detect the relationship between miR-140-5p and NCKAP1.

Results. The expression of miR-140-5p was suppressed in blood vessel of aortic dissection patients, and the levels of NCKAP1 in those tissues were upregulated. Overexpression of miR-140-5p inhibited the proliferation, migration and invasion of vascular smooth muscle cells. miR-140-5p targeted and suppressed the expression of NCKAP1.

Conclusions. miR-140-5p repressed the proliferation, migration and invasion of vascular smooth muscle cells by targeting and inhibiting the expression of NCKAP1. Furthermore, the results of our study suggest new strategies and targets for the clinical treatment of arterial dissection.

Abstract

Introduction. The occurrence of aortic dissection is related to the proliferation and metastasis of vascular smooth muscle cells. In our present study, we found that the expression of miR-140-5p was inhibited in the wall of abdominal aorta of aortic dissection patients. However, the mechanism of miR-140-5p in the development of aortic dissection is unclear.

Material and methods. We detected the expression of miR-140-5p and NCK Associated Protein 1 (NCKAP1) in blood vessel of aortic dissection patients and normal people by PCR. Next, we established the miR-140-5p overexpression and miR-140-5p inhibition vascular smooth muscle cells (CRL-1999 cells). The BrdU assays, wound healing assays and transwell assays were performed to detect the proliferation and invasion ability of these cells. Finally, luciferase reporter assay was performed to detect the relationship between miR-140-5p and NCKAP1.

Results. The expression of miR-140-5p was suppressed in blood vessel of aortic dissection patients, and the levels of NCKAP1 in those tissues were upregulated. Overexpression of miR-140-5p inhibited the proliferation, migration and invasion of vascular smooth muscle cells. miR-140-5p targeted and suppressed the expression of NCKAP1.

Conclusions. miR-140-5p repressed the proliferation, migration and invasion of vascular smooth muscle cells by targeting and inhibiting the expression of NCKAP1. Furthermore, the results of our study suggest new strategies and targets for the clinical treatment of arterial dissection.

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Keywords

aortic dissection; miR-140-5p; NCKAP1; vascular smooth muscle cells; proliferation; invasion

About this article
Title

miR-140-5p inhibits the proliferation, migration and invasion of vascular smooth muscle cells by suppressing the expression of NCKAP1

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 59, No 1 (2021)

Article type

Original paper

Pages

22-29

Published online

2021-02-09

DOI

10.5603/FHC.a2021.0003

Pubmed

33560516

Bibliographic record

Folia Histochem Cytobiol 2021;59(1):22-29.

Keywords

aortic dissection
miR-140-5p
NCKAP1
vascular smooth muscle cells
proliferation
invasion

Authors

Qing Ma
Jiancheng Liu
Chunbo Li
Dong Wang

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