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Vol 58, No 3 (2020)
Original paper
Submitted: 2020-02-03
Accepted: 2020-09-13
Published online: 2020-09-22
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The Light/Dark cycle disruption affects hepatic function both in metabolic parameters and tissue structure in a nocturnal desert rodent: Gerbillus tarabuli

Amina Derbouz Rouibate12, Nadir Benhafri12, Saliha Ouali-Hassenaoui1, Aicha Dekar-Madoui1
DOI: 10.5603/FHC.a2020.0021
·
Pubmed: 32960973
·
Folia Histochem Cytobiol 2020;58(3):182-197.
Affiliations
  1. USTHB, Faculty of Biological Sciences, Laboratory of Biology and Physiology of Organisms, Neurobiology Team, USTHB, BP 32, El Alia, Bab Ezzouar, 16111 Algiers, Algeria
  2. Dr Yahia Fares University of Medea, Faculty of Sciences, 26000 medea, Algeria

open access

Vol 58, No 3 (2020)
ORIGINAL PAPERS
Submitted: 2020-02-03
Accepted: 2020-09-13
Published online: 2020-09-22

Abstract

Introduction. Biological rhythms, such as Light/Dark (LD) cycles, are an integral component of virtually all aspects of life. These rhythms are controlled in large part by circadian clocks, allowing the organism to adapt its internal rhythmic metabolism to changes in the external environment created by daily fluctuations in the LD cycle. Therefore, changes in the daily duration of the lighting could lead to adverse health consequences. The aim of the study was to investigate, in a nocturnal desert rodent, Gerbillus tarabuli, the effects of the LD cycle disruption on the structure of the hepatic tissue and the content of carbohydrate and lipid parameters as indicators of metabolic state. Material and methods. The present study was conducted on two gerbil groups: control group was exposed to a standard lighting cycle (LD: 12:12), and the shifted group was subjected to a chronic disrupted LD cycle, alternating a standard cycle (LD: 12:12) with a modified cycle (LD: 20:4), i.e., the light phase of 24-h cycle was prolonged by 8 h on every second day during a period of 12 weeks. We used: (i) routine histology and histochemical staining for tissue analysis; (ii) immunohistochemistry (IHC) for MPO detection; (iii) biochemical methods for hepatic glycogen and lipids extraction and quantification. Blood metabolic parameters were assessed by enzymatic methods. Results. Our structural results indicate in the shifted group an alteration of tissue architecture, showing widely scattered inflammatory foci with many dilated sinusoids and prominent leukocyte infiltration with connective fibrotic extension. IHC revealed also increased hepatic myeloperoxidase (MPO) expression confirming neutrophils’ presence. In parallel, the histochemical study revealed a strong depletion of hepatocytic glycogen and lipid inclusions; these observations were also supported by the measurements of glycogen and total lipids in extracted tissue indicating a reduction in liver content. These results were accompanied by a decrease in body weight relative to the reduction of food intake, as well as hyperglycemia and some alterations in serum lipid parameters (triglycerides and cholesterol) suggesting a metabolic disturbance. Conclusion. We conclude that a phase difference between the endogenous activity rhythm of the species and the daily cycle of illumination has a strong impact on the liver morphology as well as on the metabolic activity of liver cells.

Abstract

Introduction. Biological rhythms, such as Light/Dark (LD) cycles, are an integral component of virtually all aspects of life. These rhythms are controlled in large part by circadian clocks, allowing the organism to adapt its internal rhythmic metabolism to changes in the external environment created by daily fluctuations in the LD cycle. Therefore, changes in the daily duration of the lighting could lead to adverse health consequences. The aim of the study was to investigate, in a nocturnal desert rodent, Gerbillus tarabuli, the effects of the LD cycle disruption on the structure of the hepatic tissue and the content of carbohydrate and lipid parameters as indicators of metabolic state. Material and methods. The present study was conducted on two gerbil groups: control group was exposed to a standard lighting cycle (LD: 12:12), and the shifted group was subjected to a chronic disrupted LD cycle, alternating a standard cycle (LD: 12:12) with a modified cycle (LD: 20:4), i.e., the light phase of 24-h cycle was prolonged by 8 h on every second day during a period of 12 weeks. We used: (i) routine histology and histochemical staining for tissue analysis; (ii) immunohistochemistry (IHC) for MPO detection; (iii) biochemical methods for hepatic glycogen and lipids extraction and quantification. Blood metabolic parameters were assessed by enzymatic methods. Results. Our structural results indicate in the shifted group an alteration of tissue architecture, showing widely scattered inflammatory foci with many dilated sinusoids and prominent leukocyte infiltration with connective fibrotic extension. IHC revealed also increased hepatic myeloperoxidase (MPO) expression confirming neutrophils’ presence. In parallel, the histochemical study revealed a strong depletion of hepatocytic glycogen and lipid inclusions; these observations were also supported by the measurements of glycogen and total lipids in extracted tissue indicating a reduction in liver content. These results were accompanied by a decrease in body weight relative to the reduction of food intake, as well as hyperglycemia and some alterations in serum lipid parameters (triglycerides and cholesterol) suggesting a metabolic disturbance. Conclusion. We conclude that a phase difference between the endogenous activity rhythm of the species and the daily cycle of illumination has a strong impact on the liver morphology as well as on the metabolic activity of liver cells.

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Keywords

Gerbillus tarabuli; liver, photic desynchronization; liver structure; metabolism; myeloperoxidase

About this article
Title

The Light/Dark cycle disruption affects hepatic function both in metabolic parameters and tissue structure in a nocturnal desert rodent: Gerbillus tarabuli

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 58, No 3 (2020)

Article type

Original paper

Pages

182-197

Published online

2020-09-22

Page views

1127

Article views/downloads

899

DOI

10.5603/FHC.a2020.0021

Pubmed

32960973

Bibliographic record

Folia Histochem Cytobiol 2020;58(3):182-197.

Keywords

Gerbillus tarabuli
liver
photic desynchronization
liver structure
metabolism
myeloperoxidase

Authors

Amina Derbouz Rouibate
Nadir Benhafri
Saliha Ouali-Hassenaoui
Aicha Dekar-Madoui

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