open access

Vol 57, No 3 (2019)
Original paper
Submitted: 2019-03-18
Accepted: 2019-07-19
Published online: 2019-08-02
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Cytoplasmic and membranous receptor-binding cancer antigens expressed on SiSo cells (RCAS1) immunoreactivity in epithelial ovarian cancer cells represent differing biological function of RCAS1

Sebastian Szubert12, Wojciech Jozwicki3, Lukasz Wicherek2, Krzysztof Koper4
·
Pubmed: 31388982
·
Folia Histochem Cytobiol 2019;57(3):116-126.
Affiliations
  1. Clinical Department of Gynaecological Oncology, The Franciszek Lukaszczyk Oncological Center, Bydgoszcz, Poland
  2. 2nd Department of Obstetrics and Gynecology, Medical Centre of Postgraduate Education, Warsaw, Poland
  3. Department of Tumour Pathology and Pathomorphology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Torun, Poland
  4. Department of Chemotherapy, The Franciszek Lukaszczyk Oncological Center, Bydgoszcz, Poland

open access

Vol 57, No 3 (2019)
ORIGINAL PAPERS
Submitted: 2019-03-18
Accepted: 2019-07-19
Published online: 2019-08-02

Abstract

Introduction. Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a selective suppressor of the immune response that has been linked to the evasion of immune surveillance by cancer cells. However, the exact prognostic impact of RCAS1 on epithelial ovarian cancer (EOC) has not been fully elucidated. The main aim of our study was to evaluate the influence of RCAS1 immunoreactivity (RCAS1-Ir) in EOC cells and in tumor stroma cells on patient overall survival. We also focused on RCAS1-Ir and the structure of the tumor stroma.

Material and methods. RCAS1-Ir was evaluated by means of immunohistochemistry in 67 patients with EOC. We distinguished cytoplasmic and membranous immunoreactivity patterns.

Results. We found that high cytoplasmic RCAS1-Ir in cancer cells was associated with more than a two-time shortened period of overall survival. Membranous RCAS1-Ir in cancer cells, as well as in tumor stroma macrophages and fibroblasts, did not correlate with patient survival. RCAS1-Ir in the cytoplasm of cancer cells was positively correlated with the degree of tumor stroma infiltration by fibroblasts and macrophages, but not with RCAS1-Ir in these cells. On the other hand, membranous RCAS1-Ir in cancer cells was positively correlated with RCAS1-Ir in fibroblasts and macrophages, but not with their quantity.

Conclusions. Due to their different impacts on patient prognosis and tumor stroma structure, it seems that cytoplasmic and membranous RCAS1-Ir in EOC cells may have different biological functions.

Abstract

Introduction. Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a selective suppressor of the immune response that has been linked to the evasion of immune surveillance by cancer cells. However, the exact prognostic impact of RCAS1 on epithelial ovarian cancer (EOC) has not been fully elucidated. The main aim of our study was to evaluate the influence of RCAS1 immunoreactivity (RCAS1-Ir) in EOC cells and in tumor stroma cells on patient overall survival. We also focused on RCAS1-Ir and the structure of the tumor stroma.

Material and methods. RCAS1-Ir was evaluated by means of immunohistochemistry in 67 patients with EOC. We distinguished cytoplasmic and membranous immunoreactivity patterns.

Results. We found that high cytoplasmic RCAS1-Ir in cancer cells was associated with more than a two-time shortened period of overall survival. Membranous RCAS1-Ir in cancer cells, as well as in tumor stroma macrophages and fibroblasts, did not correlate with patient survival. RCAS1-Ir in the cytoplasm of cancer cells was positively correlated with the degree of tumor stroma infiltration by fibroblasts and macrophages, but not with RCAS1-Ir in these cells. On the other hand, membranous RCAS1-Ir in cancer cells was positively correlated with RCAS1-Ir in fibroblasts and macrophages, but not with their quantity.

Conclusions. Due to their different impacts on patient prognosis and tumor stroma structure, it seems that cytoplasmic and membranous RCAS1-Ir in EOC cells may have different biological functions.

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Keywords

RCAS1; receptor-binding cancer antigen expressed on SiSo cells; epithelial ovarian cancer; tumor stroma; tumor microenvironment

About this article
Title

Cytoplasmic and membranous receptor-binding cancer antigens expressed on SiSo cells (RCAS1) immunoreactivity in epithelial ovarian cancer cells represent differing biological function of RCAS1

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 57, No 3 (2019)

Article type

Original paper

Pages

116-126

Published online

2019-08-02

Page views

1348

Article views/downloads

557

DOI

10.5603/FHC.a2019.0012

Pubmed

31388982

Bibliographic record

Folia Histochem Cytobiol 2019;57(3):116-126.

Keywords

RCAS1
receptor-binding cancer antigen expressed on SiSo cells
epithelial ovarian cancer
tumor stroma
tumor microenvironment

Authors

Sebastian Szubert
Wojciech Jozwicki
Lukasz Wicherek
Krzysztof Koper

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