Vol 57, No 1 (2019)
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Published online: 2019-03-26

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The impact of sedentary work on sperm nuclear DNA integrity

Kamil Gill1, Joanna Jakubik1, Michal Kups123, Aleksandra Rosiak-Gill13, Rafal Kurzawa43, Maciej Kurpisz5, Monika Fraczek5, Malgorzata Piasecka1
Pubmed: 30869154
Folia Histochem Cytobiol 2019;57(1):15-22.

Abstract

Introduction. Contemporary professional jobs that often enforce a sedentary lifestyle and are frequently associated with testicular overheat, deserve special attention with respect to male fertility potential. Interestingly, the harmful effect of testicular heat stress on sperm characteristics including nuclear DNA integrity was well characterized; however, the influence of sedentary work on sperm chromatin has not yet been documented. Therefore, our research was designed to examine the potential effects of sedentary work not only on conventional semen features but also on sperm nuclear DNA status.
Materials and methods. The study was carried out on ejaculated sperm cells obtained from men who spent ≥ 50% of their time at work (≥ 17.5 h per week) in a sedentary position (n = 152) and from men who spent < 50% of their time at work in a sedentary position (n = 102). Standard semen characteristics were assessed according to the WHO 2010 recommendations, while sperm nuclear DNA fragmentation (SDF) was evaluated using the Halosperm test.
Results. There were no significant differences in the standard semen parameters between the study groups. The groups differed only in SDF parameter. The men who spent at least 50% of their work time in a sedentary position had a higher proportion of SDF than the men who spent < 50% of their time at work in a sedentary position (median value 21.00% vs. 16.50%, respectively). The incidence of low SDF levels (related to 0–15% sperm cells with abnormal DNA dispersion) was significantly lower (27.63% vs. 45.10%), the percentage of men with high SDF levels (related to > 30%) was significantly higher (30.92% vs. 16.67%) in group of men who spent at least 50% of their work time in a sedentary positon. Furthermore, these men were more than twice as likely to have not a low SDF level (OR: 0.4648) and had more than twice the risk of having a high SDF level (OR: 2.2381) than the men in less sedentary occupations.
Conclusions. Despite lack of association between sedentary work and conventional semen characteristics our study revealed detrimental effect of seated work on sperm nuclear DNA integrity. A sedentary job doubled the risk of high levels of sperm DNA damage. The pathomechanism could be related to testicular heat stress resulting in sperm chromatin remodelling failure during spermiogenesis. Therefore, it seems reasonable to simultaneously carry out routine seminological analyses and tests assessing sperm chromatin status while diagnosing male infertility.

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References

  1. Inhorn MC, Patrizio P. Infertility around the globe: new thinking on gender, reproductive technologies and global movements in the 21st century. Hum Reprod Update. 2015; 21(4): 411–426.
  2. Pan MM, Hockenberry MS, Kirby EW, et al. Male Infertility Diagnosis and Treatment in the Era of In Vitro Fertilization and Intracytoplasmic Sperm Injection. Med Clin North Am. 2018; 102(2): 337–347.
  3. Uppangala S, Pudakalakatti S, D'souza F, et al. Influence of sperm DNA damage on human preimplantation embryo metabolism. Reprod Biol. 2016; 16(3): 234–241.
  4. Vaziri MH, Sadighi Gilani MA, Kavousi A, et al. The Relationship between Occupation and Semen Quality. Int J Fertil Steril. 2011; 5(2): 66–71.
  5. Agarwal A, Mulgund A, Hamada A, et al. A unique view on male infertility around the globe. Reprod Biol Endocrinol. 2015; 13: 37.
  6. Bonde JP. Male reproductive organs are at risk from environmental hazards. Asian J Androl. 2010; 12(2): 152–156.
  7. Agarwal A, Cho CL, Majzoub A, et al. The Society for Translational Medicine: clinical practice guidelines for sperm DNA fragmentation testing in male infertility. Transl Androl Urol. 2017; 6(Suppl 4): S720–S733.
  8. Kazerooni T, Asadi N, Jadid L, et al. Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet. 2009; 26(11-12): 591–596.
  9. Rex AS, Aagaard J, Fedder J. DNA fragmentation in spermatozoa: a historical review. Andrology. 2017; 5(4): 622–630.
  10. Sharma R, Agarwal A, Rohra VK, et al. Effects of increased paternal age on sperm quality, reproductive outcome and associated epigenetic risks to offspring. Reprod Biol Endocrinol. 2015; 13: 35.
  11. Yatsenko AN, Turek PJ. Reproductive genetics and the aging male. J Assist Reprod Genet. 2018; 35(6): 933–941.
  12. Varghese AC, Tan G, Chan P, et al. Clinical usefulness of sperm DNA fragmentation testing. Transl Androl Urol. 2017; 6(Suppl 4): S484–S487.
  13. Cho CL, Agarwal A. Role of sperm DNA fragmentation in male factor infertility: A systematic review. Arab J Urol. 2018; 16(1): 21–34.
  14. Durairajanayagam D. Lifestyle causes of male infertility. Arab J Urol. 2018; 16(1): 10–20.
  15. Mir J, Franken D, Andrabi SW, et al. Impact of weight loss on sperm DNA integrity in obese men. Andrologia. 2018 [Epub ahead of print].
  16. Wdowiak A, Wdowiak A, Bakalczuk S. Relationship between alcohol consumption and sperm nuclear dna fragmentation and pregnancy. Post Androl Online. 2016; 3(1): 14–21.
  17. Fraczek M, Kurpisz M. Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen. Folia Histochem Cytobiol. 2015; 53(3): 201–217.
  18. Boggia B, Carbone U, Farinaro E, et al. Effects of working posture and exposure to traffic pollutants on sperm quality. J Endocrinol Invest. 2009; 32(5): 430–434.
  19. Figà-Talamanca I, Cini C, Varricchio GC, et al. Effects of prolonged autovehicle driving on male reproduction function: a study among taxi drivers. Am J Ind Med. 1996; 30(6): 750–758.
  20. Hjollund NH, Bonde JP, Jensen TK, et al. Diurnal scrotal skin temperature and semen quality. The Danish First Pregnancy Planner Study Team. Int J Androl. 2000; 23(5): 309–318.
  21. Rao M, Xia W, Yang J, et al. Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression. Andrology. 2016; 4(6): 1054–1063.
  22. Durairajanayagam D, Sharma RK, du Plessis SS et al. Testicular Heat Stress and Sperm Quality. In: du Plessis S, Agarwal A, Sabanegh, Jr. E, eds. Male Infertility. Springer, New York; 2014:105–225. doi:10.1007/978-1-4939-1040-3_8.
  23. Durairajanayagam D, Agarwal A, Ong C. Causes, effects and molecular mechanisms of testicular heat stress. Reprod Biomed Online. 2015; 30(1): 14–27.
  24. Joubert DM. Professional Driving and Adverse Reproductive Outcomes: The Evidence to Date and Research Challenges. The Open Occupational Health & Safety Journal. 2009; 1: 1–6.
  25. Støy J, Hjøllund NH, Mortensen JT, et al. Semen quality and sedentary work position. Int J Androl. 2004; 27(1): 5–11.
  26. World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th ed. World Health Organization Press, Geneva 2010.
  27. Abdelbaki SA, Sabry JH, Al-Adl AM, et al. The impact of coexisting sperm DNA fragmentation and seminal oxidative stress on the outcome of varicocelectomy in infertile patients: A prospective controlled study. Arab J Urol. 2017; 15(2): 131–139.
  28. Al Omrani B, Al Eisa N, Javed M, et al. Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome. Reprod Biol Endocrinol. 2018; 16(1): 49.
  29. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl. 2002; 23(1): 25–43.
  30. Leach M, Aitken RJ, Sacks G. Sperm DNA fragmentation abnormalities in men from couples with a history of recurrent miscarriage. Aust N Z J Obstet Gynaecol. 2015; 55(4): 379–383.
  31. Altman DG. Comparing groups - categorical data. In. Altman DG ed.Practial statistics for medical research. Chapman & Hall: London. ; 1991: 229–279.
  32. World Health Organization. Obesity: preventing and managing the global epidemic: report of a WHO consultation. World Health Organization Press, Geneva 2000.
  33. Magnusdottir EV, Thorsteinsson T, Thorsteinsdottir S, et al. Persistent organochlorines, sedentary occupation, obesity and human male subfertility. Hum Reprod. 2005; 20(1): 208–215.
  34. De Fleurian G, Perrin J, Ecochard R, et al. Occupational exposures obtained by questionnaire in clinical practice and their association with semen quality. J Androl. 2009; 30(5): 566–579.
  35. Bach PVu, Schlegel PN. Sperm DNA damage and its role in IVF and ICSI. Basic Clin Androl. 2016; 26: 15.
  36. Evenson DP. Evaluation of sperm chromatin structure and DNA strand breaks is an important part of clinical male fertility assessment. Transl Androl Urol. 2017; 6(Suppl 4): S495–S500.
  37. Panner Selvam MK, Agarwal A. A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment. Arab J Urol. 2018; 16(1): 65–76.
  38. Koskelo R, Zaproudina N, Vuorikari K. High scrotal temperatures and chairs in the pathophysiology of poor semen quality. Pathophysiology. 2005; 11(4): 221–224.
  39. Bujan L, Daudin M, Charlet JP, et al. Increase in scrotal temperature in car drivers. Hum Reprod. 2000; 15(6): 1355–1357.
  40. Sergerie M, Mieusset R, Croute F, et al. High risk of temporary alteration of semen parameters after recent acute febrile illness. Fertil Steril. 2007; 88(4): 970.e1–970.e7.
  41. Santiso R, Tamayo M, Gosálvez J, et al. DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide. Mutat Res. 2012; 734(1-2): 41–49.
  42. Evenson DP, Wixon R, Evenson D, et al. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reprod Biomed Online. 2006; 12(4): 466–472.
  43. Sheskin DJ. Measures of association for r χ c contingency tables In: Sheskin DJ ed. Handbook of Parametric and Nonparametric Statistical Procedures. Chapman & Hall: Boca Raton. ; 2000: 655–672.



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