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Search of reference biomarkers reflecting orbital tissue remodeling in the course of Graves’ orbitopathy
Affiliations- Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Bialystok, Poland
- Department of Paediatric Ophthalmology and Strabismus, Medical University of Bialystok, Bialystok, Poland
- Department of Ophthalmology, Ludwik Rydygier District Hospital in Suwalki, Poland
- Department of Nuclear Medicine, Medical University of Bialystok, Bialystok, Poland
- Department of Immunology, Laboratory Medical Immunology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands
- Molecular Ophthalmology, Department of Ophthalmology, University Hospital Essen/University of Duisburg-Essen, Essen, Germany
- Department of Statistics and Medical Informatics, Medical University of Bialystok, Bialystok, Poland
- Department of Population Medicine and Civilization Diseases Prevention, Medical University of Bialystok, Bialystok, Poland
- Department of Rehabilitation, Medical University of Bialystok, Bialystok, Poland
- Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland
- Department of Experimental Pharmacology, Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
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Abstract
Introduction. Graves’ orbitopathy (GO) is a complication in Graves’ disease (GD) that causes disfigurement and sometimes blindness. The pathogenesis of GO remains unknown, while its symptoms demonstrate dependence between the thyroid gland and the orbit. The ongoing inflammatory process in retrobulbar tissue results in its remodeling characterized by increased volume of the orbital contents involving adipose tissue, with fibrosis and adipogenesis as predominant features. This study was aimed at the immunohistochemical verification of potential contribution and correlation between orbital expressions of IGF-1R, CD34, Foxp-3, PPAR-γ and CD4, CD68, TGF-β, FGF-β in severe and mild (long-lasting) GO. Material and methods. Forty-one orbital tissue specimens — 22 patients with severe GO, 9 patients with mild GO and 10 patients undergoing blepharoplasty as a control group — were processed by routine immunohistochemistry. Results. Increased IGF-1R, CD34 and Foxp-3 expression was found in both severe and mild GO, yet a significant correlation between CD34 and CD4, CD68, TGF-β, FGF-β expressions was observed in long-lasting GO. Conclusions. CD34 expression is proposed to be the marker of orbital tissue remodeling in the course of mild GO.
Abstract
Introduction. Graves’ orbitopathy (GO) is a complication in Graves’ disease (GD) that causes disfigurement and sometimes blindness. The pathogenesis of GO remains unknown, while its symptoms demonstrate dependence between the thyroid gland and the orbit. The ongoing inflammatory process in retrobulbar tissue results in its remodeling characterized by increased volume of the orbital contents involving adipose tissue, with fibrosis and adipogenesis as predominant features. This study was aimed at the immunohistochemical verification of potential contribution and correlation between orbital expressions of IGF-1R, CD34, Foxp-3, PPAR-γ and CD4, CD68, TGF-β, FGF-β in severe and mild (long-lasting) GO. Material and methods. Forty-one orbital tissue specimens — 22 patients with severe GO, 9 patients with mild GO and 10 patients undergoing blepharoplasty as a control group — were processed by routine immunohistochemistry. Results. Increased IGF-1R, CD34 and Foxp-3 expression was found in both severe and mild GO, yet a significant correlation between CD34 and CD4, CD68, TGF-β, FGF-β expressions was observed in long-lasting GO. Conclusions. CD34 expression is proposed to be the marker of orbital tissue remodeling in the course of mild GO.
Keywords
Graves’ orbitopathy; CD34; IGF-1R; PPAR-g; CD68; TGF-b; FGF-b; Foxp-3; tissue remodeling; autoimmunity; IHC
About this articleTitle
Search of reference biomarkers reflecting orbital tissue remodeling in the course of Graves’ orbitopathy
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
37-45
Published online
2020-03-16
Page views
1555
Article views/downloads
1185
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2020;58(1):37-45.
Keywords
Graves’ orbitopathy
CD34
IGF-1R
PPAR-g
CD68
TGF-b
FGF-b
Foxp-3
tissue remodeling
autoimmunity
IHC
Authors
Przemyslaw Pawlowski
Izabela Poplawska
Janusz Mysliwiec
Willem A. Dik
Anja Eckstein
Utta Berchner-Pfannschmidt
Robert Milewski
Slawomir Lawicki
Zofia Dzieciol-Anikiej
Robert Rejdak
Joanna Reszec
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