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Effects of massage on the expression of proangiogenic markers in rat skin
Affiliations- Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland, Wroclaw, Poland
- Department of Physiotherapy, University School of Physical Education, Wroclaw, Poland
- Public Higher Medical Professional School, Opole, Poland
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Abstract
Introduction. Massage is a physiotherapeutic treatment, commonly used in both therapy and restoration of normal body functions. The aim of this work was to determine the effects of skin massage on stimulating the expression of angiogenesis-initiating factors, i.e. VEGF-A, FGF-2 (bFGF) and CD34 and on skin regeneration processes.
Material and methods. The study was conducted on 48 Buffalo strain rats, randomly divided into two groups. In the first group (M, the massaged group), massage was applied five times a week for 7 weeks. In the second study group (C, the control group), the massage was omitted. Massage consisted of spiral movements at the plantar surface of skin for 5 min on each rear extremity. The gene expression of proangiogenic factors, including VEGF-A, FGF-2, CD34 at the mRNA level was determined using real-time PCR. Immunohistochemistry was performed on paraffin sections of rat skin to determine VEGF-A, FGF-2 CD34 and Ki-67expression.
Results. An increase in mRNA expression in the skin of the rat’s rear extremity for VEGF-A and FGF-2 in the first week of the experiment was shown in the M group compared with the control rats. The upregulation of CD34 mRNA expression was also observed in the M group. We observed positive correlations between VEGF-A mRNA expression and the expression of mRNA for FGF-2 and CD34, as well as correlation between the expression of mRNA for FGF-2 and CD34. The immunohistochemical expression of VEGF-A, FGF-2 and CD34 was at a much lower level in the skin of control rats relative to the skin of massaged animals. Moreover, significantly higher immunoreactivity was shown for nuclear protein Ki-67 in epidermal cells in the M group compared with the C group.
Conclusions. Rat skin massage increased the expression of the main angiogenesis-stimulating factors and the proliferative activity of epidermal cells, which can stimulate skin regeneration and tissue repairing processes.
Abstract
Introduction. Massage is a physiotherapeutic treatment, commonly used in both therapy and restoration of normal body functions. The aim of this work was to determine the effects of skin massage on stimulating the expression of angiogenesis-initiating factors, i.e. VEGF-A, FGF-2 (bFGF) and CD34 and on skin regeneration processes.
Material and methods. The study was conducted on 48 Buffalo strain rats, randomly divided into two groups. In the first group (M, the massaged group), massage was applied five times a week for 7 weeks. In the second study group (C, the control group), the massage was omitted. Massage consisted of spiral movements at the plantar surface of skin for 5 min on each rear extremity. The gene expression of proangiogenic factors, including VEGF-A, FGF-2, CD34 at the mRNA level was determined using real-time PCR. Immunohistochemistry was performed on paraffin sections of rat skin to determine VEGF-A, FGF-2 CD34 and Ki-67expression.
Results. An increase in mRNA expression in the skin of the rat’s rear extremity for VEGF-A and FGF-2 in the first week of the experiment was shown in the M group compared with the control rats. The upregulation of CD34 mRNA expression was also observed in the M group. We observed positive correlations between VEGF-A mRNA expression and the expression of mRNA for FGF-2 and CD34, as well as correlation between the expression of mRNA for FGF-2 and CD34. The immunohistochemical expression of VEGF-A, FGF-2 and CD34 was at a much lower level in the skin of control rats relative to the skin of massaged animals. Moreover, significantly higher immunoreactivity was shown for nuclear protein Ki-67 in epidermal cells in the M group compared with the C group.
Conclusions. Rat skin massage increased the expression of the main angiogenesis-stimulating factors and the proliferative activity of epidermal cells, which can stimulate skin regeneration and tissue repairing processes.
Keywords
rat; skin; massage; VEGF-A; FGF-2; CD34; Ki-67; qPCR; IHC
About this articleTitle
Effects of massage on the expression of proangiogenic markers in rat skin
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
83-91
Published online
2018-05-14
Page views
2808
Article views/downloads
1905
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2018;56(2):83-91.
Keywords
rat
skin
massage
VEGF-A
FGF-2
CD34
Ki-67
qPCR
IHC
Authors
Katarzyna Ratajczak-Wielgomas
Krzysztof Kassolik
Jedrzej Grzegrzolka
Tomasz Halski
Aleksandra Piotrowska
Katarzyna Mieszala
Iwona Wilk
Marzenna Podhorska-Okolow
Piotr Dziegiel
Waldemar Andrzejewski
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