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Vol 56, No 3 (2018)
Original paper
Submitted: 2017-10-22
Accepted: 2018-07-26
Published online: 2018-08-29
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Matrix metalloproteinases-2, -7 and tissue metalloproteinase inhibitor-1 expression in human endometrium

Barbara Grzechocinska1, Filip A. Dabrowski1, Anna Cyganek1, Marcin Chlebus2, Christopher Kobierzycki3, Lukasz Michalowski4, Barbara Gornicka4, Miroslaw Wielgos1
DOI: 10.5603/FHC.a2018.0017
·
Pubmed: 30187906
·
Folia Histochem Cytobiol 2018;56(3):133-140.
Affiliations
  1. Ist Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland
  2. Department of Quantitative Finance, Faculty of Economic Sciences, University of Warsaw, Warsaw, Poland, Warsaw, Poland
  3. Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland, Wroclaw, Poland
  4. Department of Pathology, Medical University of Warsaw, Warsaw, Poland

open access

Vol 56, No 3 (2018)
ORIGINAL PAPERS
Submitted: 2017-10-22
Accepted: 2018-07-26
Published online: 2018-08-29

Abstract

Introduction. Endometrium undergoes regular, cyclic tissue remodeling mostly associated to the endocrine system status. It is well-known fact that steroid hormones are strongly responsible for changes in endometrium. The precise mechanism of their action is still under investigation. The aim of the study was to evaluate the expression of metalloproteinases 2 and 7 (MMP-2, -7) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in human endometrium in relation to serum concentrations of estradiol and progesterone during different phases of menstrual cycle.
Material and methods. The study material consisted of 52 biopsy samples; 12 obtained in the proliferative phase, 11 in the secretory phase and 29 during menstruation. Expression of MMP-2, MMP-7 and TIMP-1 was assessed by immunohistochemistry. Serum concentrations of estradiol and progesterone at time of biopsy were evaluated by immunochemistry assay. Results of the study were statistically assessed by linear regression model.
Results. Increased serum concentration of estradiol was associated with increased MMP-2 expression in proliferative phase but decreased in secretory phase and during menstruation. No significant relationship was found between progesterone concentration and MMP-2 expression. Moreover, no difference in the expression of MMP-7 and TIMP-1 in the endometrium in relation to hormone levels and menstrual cycle phases were observed.
Conclusions. The results of the study indicate that estradiol influence MMP-2 expression in the endometrium depends on the phase of menstrual cycle. Such relationships were not found for MMP-7 and TIMP-1 and further tests clarifying association between estradiol and MMPs are needed.
 

Abstract

Introduction. Endometrium undergoes regular, cyclic tissue remodeling mostly associated to the endocrine system status. It is well-known fact that steroid hormones are strongly responsible for changes in endometrium. The precise mechanism of their action is still under investigation. The aim of the study was to evaluate the expression of metalloproteinases 2 and 7 (MMP-2, -7) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in human endometrium in relation to serum concentrations of estradiol and progesterone during different phases of menstrual cycle.
Material and methods. The study material consisted of 52 biopsy samples; 12 obtained in the proliferative phase, 11 in the secretory phase and 29 during menstruation. Expression of MMP-2, MMP-7 and TIMP-1 was assessed by immunohistochemistry. Serum concentrations of estradiol and progesterone at time of biopsy were evaluated by immunochemistry assay. Results of the study were statistically assessed by linear regression model.
Results. Increased serum concentration of estradiol was associated with increased MMP-2 expression in proliferative phase but decreased in secretory phase and during menstruation. No significant relationship was found between progesterone concentration and MMP-2 expression. Moreover, no difference in the expression of MMP-7 and TIMP-1 in the endometrium in relation to hormone levels and menstrual cycle phases were observed.
Conclusions. The results of the study indicate that estradiol influence MMP-2 expression in the endometrium depends on the phase of menstrual cycle. Such relationships were not found for MMP-7 and TIMP-1 and further tests clarifying association between estradiol and MMPs are needed.
 

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Keywords

metalloproteinase; MMP; tissue inhibitor of metalloproteinase; TIMP; endometrium; estradiol; progesterone

About this article
Title

Matrix metalloproteinases-2, -7 and tissue metalloproteinase inhibitor-1 expression in human endometrium

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 56, No 3 (2018)

Article type

Original paper

Pages

133-140

Published online

2018-08-29

Page views

1790

Article views/downloads

1433

DOI

10.5603/FHC.a2018.0017

Pubmed

30187906

Bibliographic record

Folia Histochem Cytobiol 2018;56(3):133-140.

Keywords

metalloproteinase
MMP
tissue inhibitor of metalloproteinase
TIMP
endometrium
estradiol
progesterone

Authors

Barbara Grzechocinska
Filip A. Dabrowski
Anna Cyganek
Marcin Chlebus
Christopher Kobierzycki
Lukasz Michalowski
Barbara Gornicka
Miroslaw Wielgos

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