open access

Vol 55, No 4 (2017)
Original paper
Submitted: 2017-08-28
Accepted: 2017-12-09
Published online: 2017-12-20
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Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Jamal Nourinezhad1, Piotr Podlasz2, Krzysztof Wasowicz3
DOI: 10.5603/FHC.a2017.0021
·
Pubmed: 29297566
·
Folia Histochem Cytobiol 2017;55(4):221-229.
Affiliations
  1. Division of Anatomy and Embryology, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran, Islamic Republic Of
  2. Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland
  3. Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland

open access

Vol 55, No 4 (2017)
ORIGINAL PAPERS
Submitted: 2017-08-28
Accepted: 2017-12-09
Published online: 2017-12-20

Abstract

Introduction. Greater splanchnic nerve (GSN) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. Despite its importance, the immunohistochemical features of the porcine GSN neurons have not yet been examined. Therefore, the aim of the study was to investigate the neurochemistry of the porcine GSN neurons and to compare their neurochemical coding with those of the paravertebral and prevertebral ganglia.

Material and methods. Four gilts of Large White Polish breed were examined in this study. Antibodies to tyrosine hydroxylase (TH), dopamine b-hydroxylase (DBH), choline acetyltransferase (ChAT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), methionine-enkephalin (MET), calcitonin gene-related peptide (CGRP), and substance P (SP) were used for immunohistochemical detection of classical neurotransmitters marker enzymes and neuropeptides in neuronal cell bodies of the GSN.

Results. Double-labeling immunofluorescence revealed that virtually all GSN neurons exhibited the presence of catecholamine-synthesizing enzymes (TH/DBH-positive) and subpopulations of neurons contained immunoreactivity to NPY, VIP, SOM, GAL and MET. However, CGRP and SP-immunoreactivity were not observed in neuronal somata.

Conclusions. Our data strongly suggest that the general immunohistochemical characterization of ganglion cells in the porcine greater splanchnic nerve is similar to that of the prevertebral ganglia (e.g. celiacomesenteric ganglion).

Abstract

Introduction. Greater splanchnic nerve (GSN) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. Despite its importance, the immunohistochemical features of the porcine GSN neurons have not yet been examined. Therefore, the aim of the study was to investigate the neurochemistry of the porcine GSN neurons and to compare their neurochemical coding with those of the paravertebral and prevertebral ganglia.

Material and methods. Four gilts of Large White Polish breed were examined in this study. Antibodies to tyrosine hydroxylase (TH), dopamine b-hydroxylase (DBH), choline acetyltransferase (ChAT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), methionine-enkephalin (MET), calcitonin gene-related peptide (CGRP), and substance P (SP) were used for immunohistochemical detection of classical neurotransmitters marker enzymes and neuropeptides in neuronal cell bodies of the GSN.

Results. Double-labeling immunofluorescence revealed that virtually all GSN neurons exhibited the presence of catecholamine-synthesizing enzymes (TH/DBH-positive) and subpopulations of neurons contained immunoreactivity to NPY, VIP, SOM, GAL and MET. However, CGRP and SP-immunoreactivity were not observed in neuronal somata.

Conclusions. Our data strongly suggest that the general immunohistochemical characterization of ganglion cells in the porcine greater splanchnic nerve is similar to that of the prevertebral ganglia (e.g. celiacomesenteric ganglion).

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Keywords

greater splanchnic nerve; neurons; neurotransmitters; pig; immunohistochemistry

About this article
Title

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 55, No 4 (2017)

Article type

Original paper

Pages

221-229

Published online

2017-12-20

DOI

10.5603/FHC.a2017.0021

Pubmed

29297566

Bibliographic record

Folia Histochem Cytobiol 2017;55(4):221-229.

Keywords

greater splanchnic nerve
neurons
neurotransmitters
pig
immunohistochemistry

Authors

Jamal Nourinezhad
Piotr Podlasz
Krzysztof Wasowicz

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