open access

Vol 55, No 4 (2017)
Original paper
Submitted: 2017-05-21
Accepted: 2018-01-23
Published online: 2018-02-05
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Immunoexpression of IgA receptors (CD89, CD71) in dermatitis herpetiformis

Justyna Gornowicz-Porowska1, Agnieszka Seraszek-Jaros2, Monika Bowszyc-Dmochowska3, Elzbieta Kaczmarek2, Marian Dmochowski4
DOI: 10.5603/FHC.a2017.0024
·
Pubmed: 29417981
·
Folia Histochem Cytobiol 2017;55(4):212-220.
Affiliations
  1. Autoimmune Blistering Dermatoses Section, Department of Dermatology, Poznan University of Medical Sciences, Poznan, Poland
  2. Department of Bioinformatics and Computational Biology, Poznan University of Medical Sciences, 79 Dabrowskiego Street, 60-529 Poznan, Poland
  3. Cutaneous Histopathology and Immunopathology Section, Department of Dermatology, Poznan University of Medical Sciences, 49 Przybyszewskiego Street, 60-355 Poznan, Pitcairn
  4. Autoimmune Blistering Dermatoses Section, Department of Dermatology, Poznan University of Medical Sciences, 49 Przybyszewskiego Street, 60-355 Poznan, Poland

open access

Vol 55, No 4 (2017)
ORIGINAL PAPERS
Submitted: 2017-05-21
Accepted: 2018-01-23
Published online: 2018-02-05

Abstract

Introduction. The role of IgA receptors in dermatitis herpetiformis (DH) pathogenesis is still unknown. CD89 and CD71 may be associated with immune response during DH development. The purpose of this study was to perform semiquantitative analysis of simultaneous immunoexpression of CD89 and CD71 in DH and IgA/neutrophil-mediated non-DH dermatoses (IgAN) in relation to specific IgA autoantibodies/antibodies (tissue and epidermal transglutaminases, nonapeptides of gliadin — eTG/tTG/npG) as well neutrophil activation via the release of neutrophil elastase (NE).

Material and methods. In total, 48 patients were studied. The study was conducted on skin lesions and sera obtained from DH and IgAN patients. DH and IgAN served as mutually positive control groups. We used immunohistochemical technique with semiquantitative digital morphometry and ELISA to measure serum levels of anti-eTG/tTG/npG IgA.

Results. CD89 showed a significantly higher expression in DH than in IgAN. CD71 was overexpressed in DH and IgAN. CD89 immunoexpression correlated negatively with CD71 in IgAN. A positive correlation was revealed between CD89 immunoexpression and anti-npG IgA in DH. No statistically significant correlations were found in DH between the CD89/CD71 and NE immunoexpression, between CD71 immunoexpression and anti-tTG/eTG/npG IgA, or between CD89 immunoexpression and anti-eTG/tTG IgA serum levels.

Conclusions. CD89 is probably a key IgA Fc receptor in DH development, where it is associated with immune response to gluten. CD71 may be linked with inflammation in DH and IgAN. We suggest that interaction between CD89 and CD71 can modulate the inflammation in IgAN.

Abstract

Introduction. The role of IgA receptors in dermatitis herpetiformis (DH) pathogenesis is still unknown. CD89 and CD71 may be associated with immune response during DH development. The purpose of this study was to perform semiquantitative analysis of simultaneous immunoexpression of CD89 and CD71 in DH and IgA/neutrophil-mediated non-DH dermatoses (IgAN) in relation to specific IgA autoantibodies/antibodies (tissue and epidermal transglutaminases, nonapeptides of gliadin — eTG/tTG/npG) as well neutrophil activation via the release of neutrophil elastase (NE).

Material and methods. In total, 48 patients were studied. The study was conducted on skin lesions and sera obtained from DH and IgAN patients. DH and IgAN served as mutually positive control groups. We used immunohistochemical technique with semiquantitative digital morphometry and ELISA to measure serum levels of anti-eTG/tTG/npG IgA.

Results. CD89 showed a significantly higher expression in DH than in IgAN. CD71 was overexpressed in DH and IgAN. CD89 immunoexpression correlated negatively with CD71 in IgAN. A positive correlation was revealed between CD89 immunoexpression and anti-npG IgA in DH. No statistically significant correlations were found in DH between the CD89/CD71 and NE immunoexpression, between CD71 immunoexpression and anti-tTG/eTG/npG IgA, or between CD89 immunoexpression and anti-eTG/tTG IgA serum levels.

Conclusions. CD89 is probably a key IgA Fc receptor in DH development, where it is associated with immune response to gluten. CD71 may be linked with inflammation in DH and IgAN. We suggest that interaction between CD89 and CD71 can modulate the inflammation in IgAN.

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Keywords

dermatitis herpetiformis; IgAN; Fc-alpha receptor; CD71; CD89; IHC

About this article
Title

Immunoexpression of IgA receptors (CD89, CD71) in dermatitis herpetiformis

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 55, No 4 (2017)

Article type

Original paper

Pages

212-220

Published online

2018-02-05

DOI

10.5603/FHC.a2017.0024

Pubmed

29417981

Bibliographic record

Folia Histochem Cytobiol 2017;55(4):212-220.

Keywords

dermatitis herpetiformis
IgAN
Fc-alpha receptor
CD71
CD89
IHC

Authors

Justyna Gornowicz-Porowska
Agnieszka Seraszek-Jaros
Monika Bowszyc-Dmochowska
Elzbieta Kaczmarek
Marian Dmochowski

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