Vol 56, No 1 (2018)
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Published online: 2018-03-05

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Immunohistochemical mapping of neurotensin in the alpaca diencephalon

Manuel Lisardo Sánchez1, Arturo Mangas1, Luis Enrique Medina1, Luis Angel Aguilar2, Zaida Díaz-Cabiale3, José Angel Narváez3, Rafael Coveñas1
Pubmed: 29516445
Folia Histochem Cytobiol 2018;56(1):49-58.


Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca
diencephalon was determined by an immunohistochemical technique.
Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply
anesthetized animals were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were stained by
a standard immunohistochemical method.
Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate
density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic
areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic
nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the
thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus
a high density was in general observed in the whole structure.
Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca
diencephalon suggests that the peptide can be involved in many physiological actions.

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  1. Carraway RE, Leeman SE. Characterization of radioimmunoassayableneurotensin in the rat. Its differential distribution in the central nervous system, small intestine and stomach. J BiolChem 1976; 251: 7045-7052. PMID. : 993203.
  2. Cooper PE, Fernstrom MH, Rorstad OP, et al. The regional distribution of somatostatin, substance P and neurotensin in human brain. Brain Res. 1981; 218(1-2): 219–232.
  3. De León M, Coveñas R, Narváez JA, et al. Neurotensin-like immunoreactivity in the diencephalon of the adult male cat. Peptides. 1991; 12(2): 257–264.
  4. De León M, Coveñas R, Narváez JA, et al. Distribution of neurotensin-like immunoreactive cell bodies and fibers in the brainstem of the adult male cat. Peptides. 1991; 12(6): 1201–1209.
  5. Jennes L, Stumpf WE, Kalivas PW. Neurotensin: topographical distribution in rat brain by immunohistochemistry. J Comp Neurol. 1982; 210(3): 211–224.
  6. Kataoka K, Mizuno N, Frohman LA. Regioal distribution of immunoreactive neurotension in monkey brain. Brain Res Bull. 1979; 4(1): 57–60.
  7. Kobayashi RM, Brown M, Vale W. Regional distribution of neurotensin and somatostatin in rat brain. Brain Res. 1977; 126(3): 584–588.
  8. Mai JK, Triepel J, Metz J. Neurotensin in the human brain. Neuroscience. 1987; 22(2): 499–524.
  9. Sánchez ML, Vecino E, Coveñas R. Distribution of Neurotensin and Somatostatin-28 (1-12) in the Minipig Brainstem. Anat Histol Embryol. 2016; 45(4): 260–276.
  10. Triepel J, Weindl A, Heinrich D, et al. Distribution of neurotensin-immunoreactiveperikarya in the brainstem of the guinea pig. Histochemistry. 1984; 13: 229–312.
  11. Uhl GR, Kuhar MJ, Snyder SH. Neurotensin: immunohistochemical localization in rat central nervous system. Proceedings of the National Academy of Sciences. 1977; 74(9): 4059–4063.
  12. de Souza E, Aguilar LA, Díaz-Cabiale Z, et al. Mapping of neurotensin in the alpaca (Lama pacos) brainstem. Anat Histol Embryol. 2014; 43(4): 245–256.
  13. Schroeder LE, Leinninger GM. Role of central neurotensin in regulating feeding: Implications for the development and treatment of body weight disorders. Biochim Biophys Acta. 2018; 1864(3): 900–916.
  14. Kaczyńska K, Zając D, Wojciechowski P, et al. Neuropeptides and breathing in health and disease. Pulm Pharmacol Ther. 2018; 48: 217–224.
  15. Kuhre RE, Christiansen CB, Saltiel MY, et al. On the relationship between glucose absorption and glucose-stimulated secretion of GLP-1, neurotensin, and PYY from different intestinal segments in the rat. Physiol Rep. 2017; 5(23).
  16. Lénárd L, László K, Kertes E, et al. Substance P and neurotensin in the limbic system: Their roles in reinforcement and memory consolidation. Neurosci Biobehav Rev. 2018; 85: 1–20.
  17. Gandou C, Ohtani A, Senzaki K, et al. Neurotensin promotes the dendrite elongation and the dendritic spine maturation of the cerebral cortex in vitro. Neurosci Res. 2010; 66(3): 246–255.
  18. Martorana A, Martella G, D'Angelo V, et al. Neurotensin effects on N-type calcium currents among rat pallidal neurons: an electrophysiological and immunohistochemical study. Synapse. 2006; 60(5): 371–383.
  19. Kim J, Napier D, Weiss H, et al. Neurotensin Receptor 3/Sortilin Contributes to Tumorigenesis of Neuroendocrine Tumors Through Augmentation of Cell Adhesion and Migration. Neoplasia. 2018; 20(2): 175–181.
  20. Abbaci A, Talbot H, Saada S, et al. Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis. Oncogene. 2017; 37(6): 756–767.
  21. Baum RP, Singh A, Schuchardt C, et al. Lu-3BP-227 for neurotensin receptor 1-targeted therapy of metastatic pancreatic adenocarcinoma - first clinical results. J Nucl Med. 2017 [Epub ahead of print].
  22. Bravo PW, Stewart DR, Lasley BL, et al. Hormonal indicators of pregnancy in llamas and alpacas. J Am Vet Med Assoc. 1996; 208(12): 2027–2030.
  23. Correa JE, Ratto MH, Gatica R. Superovulation in llamas (Lama glama) with pFSH and equine chorionic gonadotrophin used individually or in combination. Anim Reprod Sci. 1997; 46(3-4): 289–296.
  24. Ratto MH, Gatica R, Correa JE. Timing of mating and ovarian response in llamas (Lama glama) treated with pFSH. Anim Reprod Sci. 1997; 48(2-4): 325–330.
  25. Ratto MH, Huanca W, Singh J, et al. Local versus systemic effect of ovulation-inducing factor in the seminal plasma of alpacas. Reprod Biol Endocrinol. 2005; 3: 29.
  26. Ratto M, Huanca W, Singh J, et al. Comparison of the effect of natural mating, LH, and GnRH on interval to ovulation and luteal function in llamas. Anim Reprod Sci. 2006; 91(3-4): 299–306.
  27. Coveñas R, Mangas A, Medina LE, et al. Mapping of somatostatin-28 (1-12) in the alpaca diencephalon. J Chem Neuroanat. 2011; 42(1): 89–98.
  28. Coveñas R, Sánchez ML, Mangas A, et al. Mapping of CGRP in the alpaca diencephalon. J Chem Neuroanat. 2012; 45(1-2): 36–44.
  29. de Souza E, Yi P, Aguilar LA, et al. et al.. Mapping of leucine-enkephalin in the alpaca (Lama pacos) brainstem. In: CoveñasR, , eds. Focus on Neuropeptide Research. Trivandrum: Transworld Research Network. ; 2007: 103–114.
  30. de Souza E, Coveñas R, Yi P, et al. Mapping of CGRP in the alpaca (Lama pacos) brainstem. J Chem Neuroanat. 2008; 35(4): 346–355.
  31. Souza EDe, Sánchez M, Aguilar L, et al. Mapping of somatostatin-28 (1-12) in the alpaca (Lama pacos) brainstem. Microscopy Research and Technique. 2015; 78(5): 363–374.
  32. Manso B, Sánchez ML, Medina LE, et al. Immunohistochemical mapping of pro-opiomelanocortin- and pro-dynorphin-derived peptides in the alpaca (Lama pacos) diencephalon. J Chem Neuroanat. 2014; 59-60: 36–50.
  33. Studler JM, Kitabgi P, Tramu G, et al. Extensive co-localization of neurotensin with dopamine in rat meso-cortico-frontal dopaminergic neurons. Neuropeptides. 1988; 11(3): 95–100.
  34. Marcos P, Arroyo-Jiménez MM, Lozano G, et al. Mapping of tyrosine hydroxylase in the diencephalon of alpaca (Lama pacos) and co-distribution with somatostatin-28 (1-12). J Chem Neuroanat. 2013; 50-51: 66–74.
  35. Coveñas R, de Le, Narváez JA, et al. Anatomical distribution of beta-endorphin (1-27) in the cat brainstem: an immunocytochemical study. Anat Embryol 1999; 199: 161-167. PMID. : 9930622.
  36. Kobayashi A, Osaka T, Namba Y, et al. CGRP microinjection into the ventromedial or dorsomedial hypothalamic nucleus activates heat production. Brain Res. 1999; 827(1-2): 176–184.