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Vol 56, No 1 (2018)
Original paper
Submitted: 2017-04-20
Accepted: 2018-02-26
Published online: 2018-03-05
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Immunohistochemical mapping of neurotensin in the alpaca diencephalon

Manuel Lisardo Sánchez1, Arturo Mangas1, Luis Enrique Medina1, Luis Angel Aguilar2, Zaida Díaz-Cabiale3, José Angel Narváez3, Rafael Coveñas1
DOI: 10.5603/FHC.a2018.0003
·
Pubmed: 29516445
·
Folia Histochem Cytobiol 2018;56(1):49-58.
Affiliations
  1. Institute of Neurosciences of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems (Lab. 14), Salamanca, Spain
  2. San Ignacio de Loyola University (USIL), Faculty of Health Sciences, Lima, Peru
  3. University of Málaga, School of Medicine, Department of Physiology, Málaga, Spain

open access

Vol 56, No 1 (2018)
ORIGINAL PAPERS
Submitted: 2017-04-20
Accepted: 2018-02-26
Published online: 2018-03-05

Abstract

Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca
diencephalon was determined by an immunohistochemical technique.
Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply
anesthetized animals were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were stained by
a standard immunohistochemical method.
Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate
density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic
areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic
nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the
thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus
a high density was in general observed in the whole structure.
Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca
diencephalon suggests that the peptide can be involved in many physiological actions.

Abstract

Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca
diencephalon was determined by an immunohistochemical technique.
Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply
anesthetized animals were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were stained by
a standard immunohistochemical method.
Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate
density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic
areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic
nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the
thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus
a high density was in general observed in the whole structure.
Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca
diencephalon suggests that the peptide can be involved in many physiological actions.

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Keywords

camelid; Lama pacos; hypothalamus; thalamus; IHC

About this article
Title

Immunohistochemical mapping of neurotensin in the alpaca diencephalon

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 56, No 1 (2018)

Article type

Original paper

Pages

49-58

Published online

2018-03-05

Page views

3500

Article views/downloads

1600

DOI

10.5603/FHC.a2018.0003

Pubmed

29516445

Bibliographic record

Folia Histochem Cytobiol 2018;56(1):49-58.

Keywords

camelid
Lama pacos
hypothalamus
thalamus
IHC

Authors

Manuel Lisardo Sánchez
Arturo Mangas
Luis Enrique Medina
Luis Angel Aguilar
Zaida Díaz-Cabiale
José Angel Narváez
Rafael Coveñas

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