open access

Vol 54, No 3 (2016)
ORIGINAL PAPERS
Published online: 2016-08-16
Submitted: 2016-02-22
Accepted: 2016-07-22
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Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

Haidong Wang, Linli Xue, Yanan Li, Bingling Zhao, Tianzhi Chen, Ying Liu, Lucheng Chang, Juan Wang
DOI: 10.5603/FHC.a2016.0015
·
Pubmed: 27654014
·
Folia Histochem Cytobiol 2016;54(3):143-150.

open access

Vol 54, No 3 (2016)
ORIGINAL PAPERS
Published online: 2016-08-16
Submitted: 2016-02-22
Accepted: 2016-07-22

Abstract

Introduction. To investigate whether the membrane-associated transporter protein SLC45A2 is differentially expressed in the skin of sheep with different coat colors and to determine its correlation with coat color establishment in sheep.

Material and methods. The expression of SLC45A2 in sheep skin samples with different coat colors was qualitatively and quantitatively analyzed by PCR amplification, RT-PCR, immunohistochemical staining and Western blotting.

Results. A 193-bp SLC45A2 CDS sequence was successfully amplified from sheep skin samples with diverse coat colors. RT-PCR analysis revealed that SLC45A2 mRNA was expressed in all sheep skin samples tested, with relative expression levels of 512.74 ± 121.51 in black skin, 143.38 ± 119.31 and 1.36 ± 0.09 in black dots and white dots of piebald skin, respectively, and 1.02 ± 0.23 in white skin (p < 0.01**). Positive SLC45A2 protein bands were also detected in all skin samples by Western blot analysis, with relative expression levels of 0.85 ± ± 0.17** in black skin, 0.60 ± 0.05** and 0.34 ± 0.07 in black dots and white dots of piebald skin, respectively, and 0.20 ± 0.05 in white skin (p < 0.01**). Immunohistochemical assays revealed that SLC45A2 was expressed in the hair follicle matrix, the inner and outer root sheath, and the dermal papilla in the skin tissues with different coat colors. These patterns were quantified by optical density (OD) analysis, which yielded relative expression levels of 0.23 ± 0.11 in black skin, 0.19 ± 0.09 and 0.10 ± 0.03 in black dots and white dots of piebald skin, respectively, and 0.08 ± 0.01 in white skin (p < 0.05*).

Conclusion. SLC45A2 is detectably expressed in sheep skin of all coat colors, though at significantly different levels. SLC45A2 may participate in the establishment of coat color by regulating the synthesis and trafficking of melanin.  

Abstract

Introduction. To investigate whether the membrane-associated transporter protein SLC45A2 is differentially expressed in the skin of sheep with different coat colors and to determine its correlation with coat color establishment in sheep.

Material and methods. The expression of SLC45A2 in sheep skin samples with different coat colors was qualitatively and quantitatively analyzed by PCR amplification, RT-PCR, immunohistochemical staining and Western blotting.

Results. A 193-bp SLC45A2 CDS sequence was successfully amplified from sheep skin samples with diverse coat colors. RT-PCR analysis revealed that SLC45A2 mRNA was expressed in all sheep skin samples tested, with relative expression levels of 512.74 ± 121.51 in black skin, 143.38 ± 119.31 and 1.36 ± 0.09 in black dots and white dots of piebald skin, respectively, and 1.02 ± 0.23 in white skin (p < 0.01**). Positive SLC45A2 protein bands were also detected in all skin samples by Western blot analysis, with relative expression levels of 0.85 ± ± 0.17** in black skin, 0.60 ± 0.05** and 0.34 ± 0.07 in black dots and white dots of piebald skin, respectively, and 0.20 ± 0.05 in white skin (p < 0.01**). Immunohistochemical assays revealed that SLC45A2 was expressed in the hair follicle matrix, the inner and outer root sheath, and the dermal papilla in the skin tissues with different coat colors. These patterns were quantified by optical density (OD) analysis, which yielded relative expression levels of 0.23 ± 0.11 in black skin, 0.19 ± 0.09 and 0.10 ± 0.03 in black dots and white dots of piebald skin, respectively, and 0.08 ± 0.01 in white skin (p < 0.05*).

Conclusion. SLC45A2 is detectably expressed in sheep skin of all coat colors, though at significantly different levels. SLC45A2 may participate in the establishment of coat color by regulating the synthesis and trafficking of melanin.  

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Keywords

SLC45A2; sheep; coat color; Q-PCR; Western blot; IHC

About this article
Title

Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 54, No 3 (2016)

Pages

143-150

Published online

2016-08-16

DOI

10.5603/FHC.a2016.0015

Pubmed

27654014

Bibliographic record

Folia Histochem Cytobiol 2016;54(3):143-150.

Keywords

SLC45A2
sheep
coat color
Q-PCR
Western blot
IHC

Authors

Haidong Wang
Linli Xue
Yanan Li
Bingling Zhao
Tianzhi Chen
Ying Liu
Lucheng Chang
Juan Wang

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