open access

Vol 54, No 1 (2016)
Original paper
Submitted: 2015-09-14
Accepted: 2016-04-05
Published online: 2016-05-10
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Changes of memory B- and T-cell subsets in lupus nephritis patients

Joanna Kosalka, Bogdan Jakiela, Jacek Musial
DOI: 10.5603/FHC.a2016.0005
·
Pubmed: 27094637
·
Folia Histochem Cytobiol 2016;54(1):32-41.

open access

Vol 54, No 1 (2016)
ORIGINAL PAPERS
Submitted: 2015-09-14
Accepted: 2016-04-05
Published online: 2016-05-10

Abstract

Introduction. Renal involvement in systemic lupus erythematosus (SLE) is associated with production of antibodies to double stranded DNA, deposition of immune complexes and organ damage. These processes have been linked with abnormalities in B- and T-cell memory compartments. The aim of the study was to analyze subsets of peripheral memory B-cells and T-cells in lupus nephritis (LN) patients.

Material and methods. We used multicolor flow cytometry to analyze major memory subsets of peripheral blood B-cells (defined by CD27, IgD and CD21) and T-cells (CD45RA, CD45RO, CCR7) in 32 patients with active or inactive LN, and 23 control subjects.

Results. Lupus nephritis patients were characterized by increased percentage of immature/early-transitional B-cells (CD27-IgD+CD21-), higher frequency of activated switched memory (SM, CD27+IgD-CD21-) and exhausted memory B-cells (CD27-IgD-), and decrease in non-switched memory (NSM, CD27+IgD+) B-cells. CD21low subsets (immature and activated B-cells) were particularly expanded in patients with active disease. In both groups of LN patients we observed decline in the absolute count of NSM B-cells. It was paralleled by lymphopenia in naïve CD4+ T-cell compartment and increase in the frequency of effector memory T-cells, and these changes were more pronounced in active LN.

Conclusions. B-cell memory compartment in LN is deficient in NSM cells and during active disease it is further skewed towards SM and exhausted memory phenotypes, most likely as a cause of chronic antigenic stimulation. Parallel changes in T-helper cell subsets suggest a similar mechanism of SLE-related lymphopenia for both B-cell and T-cell compartment.

Abstract

Introduction. Renal involvement in systemic lupus erythematosus (SLE) is associated with production of antibodies to double stranded DNA, deposition of immune complexes and organ damage. These processes have been linked with abnormalities in B- and T-cell memory compartments. The aim of the study was to analyze subsets of peripheral memory B-cells and T-cells in lupus nephritis (LN) patients.

Material and methods. We used multicolor flow cytometry to analyze major memory subsets of peripheral blood B-cells (defined by CD27, IgD and CD21) and T-cells (CD45RA, CD45RO, CCR7) in 32 patients with active or inactive LN, and 23 control subjects.

Results. Lupus nephritis patients were characterized by increased percentage of immature/early-transitional B-cells (CD27-IgD+CD21-), higher frequency of activated switched memory (SM, CD27+IgD-CD21-) and exhausted memory B-cells (CD27-IgD-), and decrease in non-switched memory (NSM, CD27+IgD+) B-cells. CD21low subsets (immature and activated B-cells) were particularly expanded in patients with active disease. In both groups of LN patients we observed decline in the absolute count of NSM B-cells. It was paralleled by lymphopenia in naïve CD4+ T-cell compartment and increase in the frequency of effector memory T-cells, and these changes were more pronounced in active LN.

Conclusions. B-cell memory compartment in LN is deficient in NSM cells and during active disease it is further skewed towards SM and exhausted memory phenotypes, most likely as a cause of chronic antigenic stimulation. Parallel changes in T-helper cell subsets suggest a similar mechanism of SLE-related lymphopenia for both B-cell and T-cell compartment.

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Keywords

systemic lupus erythematosus; lupus nephritis; immune memory; B-cells; T-cells; flow cytometry

About this article
Title

Changes of memory B- and T-cell subsets in lupus nephritis patients

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 54, No 1 (2016)

Article type

Original paper

Pages

32-41

Published online

2016-05-10

DOI

10.5603/FHC.a2016.0005

Pubmed

27094637

Bibliographic record

Folia Histochem Cytobiol 2016;54(1):32-41.

Keywords

systemic lupus erythematosus
lupus nephritis
immune memory
B-cells
T-cells
flow cytometry

Authors

Joanna Kosalka
Bogdan Jakiela
Jacek Musial

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