open access

Vol 53, No 2 (2015)
ORIGINAL PAPERS
Published online: 2015-07-06
Submitted: 2015-06-23
Accepted: 2015-07-02
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Increased skeletal muscle expression of VEGF induced by massage and exercise

Waldemar Andrzejewski, Krzysztof Kassolik, Christopher Kobierzycki, Jedrzej Grzegrzolka, Katarzyna Ratajczak-Wielgomas, Karolina Jablonska, Tomasz Halski, Piotr Dziegiel, Bohdan Gworys, Marzenna Podhorska-Okolow
DOI: 10.5603/FHC.a2015.0013
·
Pubmed: 26150286
·
Folia Histochem Cytobiol 2015;53(2):145-151.

open access

Vol 53, No 2 (2015)
ORIGINAL PAPERS
Published online: 2015-07-06
Submitted: 2015-06-23
Accepted: 2015-07-02

Abstract

Introduction. Numerous investigations have been carried out to describe the role of massage in preparing for and restoring efficiency after physical exercise. Furthermore, vascular endothelial growth factor (VEGF) enhances blood vessel growth, and in effect contributes to the regeneration of tissues. Since its expression in active skeletal muscles has not been yet determined, the aim of this study was to investigate whether muscle massage performed before and during running exercise affects the expression of VEGF-A in muscles.

Material and methods. The study was carried out on 75 adult Buffalo rats subjected to running exercise training for 10 weeks. Rats were massaged prior (group PM) or during exercise (group M) or were not massaged (group C). The massage consisted of spiral movements along the plantar surface of flexor digitorum brevis muscle. After 1, 3, 5, 7 and 10 week of training, five rats from every group were anesthetized and immunohistochemistry, Western blot, and PCR analyses were performed on obtained muscle tissue to determine VEGF-A expression.

Results. After the first week of training, a significant increase of VEGF-A gene expression analyzed by qPCR in muscle tissue was observed in the PM group, whereas in the third week, the predominant growth of studied marker was seen in the M group. Increased VEGF-A expression on the protein level was observed in both massaged groups following the first week. A moderate positive correlation was found between the expression of the VEGF-A gene and protein in all experimental groups (r = 0.389).

Conclusion. Short-term repeated massage may contribute to processes of creation of new and development of already existing vascular networks in the skeletal muscle tissue during increased exercise.

Abstract

Introduction. Numerous investigations have been carried out to describe the role of massage in preparing for and restoring efficiency after physical exercise. Furthermore, vascular endothelial growth factor (VEGF) enhances blood vessel growth, and in effect contributes to the regeneration of tissues. Since its expression in active skeletal muscles has not been yet determined, the aim of this study was to investigate whether muscle massage performed before and during running exercise affects the expression of VEGF-A in muscles.

Material and methods. The study was carried out on 75 adult Buffalo rats subjected to running exercise training for 10 weeks. Rats were massaged prior (group PM) or during exercise (group M) or were not massaged (group C). The massage consisted of spiral movements along the plantar surface of flexor digitorum brevis muscle. After 1, 3, 5, 7 and 10 week of training, five rats from every group were anesthetized and immunohistochemistry, Western blot, and PCR analyses were performed on obtained muscle tissue to determine VEGF-A expression.

Results. After the first week of training, a significant increase of VEGF-A gene expression analyzed by qPCR in muscle tissue was observed in the PM group, whereas in the third week, the predominant growth of studied marker was seen in the M group. Increased VEGF-A expression on the protein level was observed in both massaged groups following the first week. A moderate positive correlation was found between the expression of the VEGF-A gene and protein in all experimental groups (r = 0.389).

Conclusion. Short-term repeated massage may contribute to processes of creation of new and development of already existing vascular networks in the skeletal muscle tissue during increased exercise.

Get Citation

Keywords

rat; physical exercise; massage; skeletal muscle; VEGF-A

About this article
Title

Increased skeletal muscle expression of VEGF induced by massage and exercise

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 53, No 2 (2015)

Pages

145-151

Published online

2015-07-06

DOI

10.5603/FHC.a2015.0013

Pubmed

26150286

Bibliographic record

Folia Histochem Cytobiol 2015;53(2):145-151.

Keywords

rat
physical exercise
massage
skeletal muscle
VEGF-A

Authors

Waldemar Andrzejewski
Krzysztof Kassolik
Christopher Kobierzycki
Jedrzej Grzegrzolka
Katarzyna Ratajczak-Wielgomas
Karolina Jablonska
Tomasz Halski
Piotr Dziegiel
Bohdan Gworys
Marzenna Podhorska-Okolow

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