open access

Vol 53, No 2 (2015)
Short Communication
Published online: 2015-07-29
Submitted: 2015-05-26
Accepted: 2015-07-07
Get Citation

Differential resistance of human embryonic stem cells and somatic cell types to hydrogen peroxide-induced genotoxicity may be dependent on innate basal intracellular ROS levels

Kumar Jayaseelan Vinoth, Jayapal Manikandan, Swaminathan Sethu, Lakshmidevi Balakrishnan, Alexis Heng, Kai Lu, Anuradha Poonepalli, Manoor Prakash Hande, Tong Cao
DOI: 10.5603/FHC.a2015.0016
·
Pubmed: 26156296
·
Folia Histochem Cytobiol 2015;53(2):169-174.

open access

Vol 53, No 2 (2015)
Short Communication
Published online: 2015-07-29
Submitted: 2015-05-26
Accepted: 2015-07-07

Abstract

Previously, we demonstrated that undifferentiated human embryonic stem cells (hESC) displayed higher resistance to oxidative and genotoxic stress compared to somatic cells, but did not further probe the underlying mechanisms. Using H2O2-induced genotoxicity as a model, this study investigated whether higher resistance of hESC to oxidative and genotoxic stress could be due to lower innate basal intracellular levels of reactive oxygen species (ROS), as compared to their differentiated fibroblastic progenies (H1F) and two other somatic cell types — human embryonic palatal mesenchymal (HEPM) cells and peripheral blood lymphocytes (PBL). Comet assay demonstrated that undifferentiated hESC consistently sustained lower levels of DNA damage upon acute exposure to H2O2 for 30 min, compared to somatic cells. DCFDA and HE staining with flow cytometry showed that undifferentiated hESC had lower innate basal intracellular levels of reactive oxygen species compared to somatic cells, which could lead to their higher resistance to genotoxic stress upon acute exposure to H2O2.

Abstract

Previously, we demonstrated that undifferentiated human embryonic stem cells (hESC) displayed higher resistance to oxidative and genotoxic stress compared to somatic cells, but did not further probe the underlying mechanisms. Using H2O2-induced genotoxicity as a model, this study investigated whether higher resistance of hESC to oxidative and genotoxic stress could be due to lower innate basal intracellular levels of reactive oxygen species (ROS), as compared to their differentiated fibroblastic progenies (H1F) and two other somatic cell types — human embryonic palatal mesenchymal (HEPM) cells and peripheral blood lymphocytes (PBL). Comet assay demonstrated that undifferentiated hESC consistently sustained lower levels of DNA damage upon acute exposure to H2O2 for 30 min, compared to somatic cells. DCFDA and HE staining with flow cytometry showed that undifferentiated hESC had lower innate basal intracellular levels of reactive oxygen species compared to somatic cells, which could lead to their higher resistance to genotoxic stress upon acute exposure to H2O2.

Get Citation

Keywords

human ESC; genotoxicity; H2O2; reactive oxygen species; comet assay; flow cytometry

About this article
Title

Differential resistance of human embryonic stem cells and somatic cell types to hydrogen peroxide-induced genotoxicity may be dependent on innate basal intracellular ROS levels

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 53, No 2 (2015)

Pages

169-174

Published online

2015-07-29

DOI

10.5603/FHC.a2015.0016

Pubmed

26156296

Bibliographic record

Folia Histochem Cytobiol 2015;53(2):169-174.

Keywords

human ESC
genotoxicity
H2O2
reactive oxygen species
comet assay
flow cytometry

Authors

Kumar Jayaseelan Vinoth
Jayapal Manikandan
Swaminathan Sethu
Lakshmidevi Balakrishnan
Alexis Heng
Kai Lu
Anuradha Poonepalli
Manoor Prakash Hande
Tong Cao

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