Folia Histochemica et Cytobiologica

open access

Vol 53, No 3 (2015)
Original paper
Submitted: 2015-04-06
Accepted: 2015-09-01
Published online: 2015-09-04
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Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Ghadeer I. Alrefaei, Nasra N. Ayuob, Soad S. Ali, Saleh Al-Karim
DOI: 10.5603/FHC.a2015.0022
·
Pubmed: 26339985
·
Folia Histochem Cytobiol 2015;53(3):259-271.

open access

Vol 53, No 3 (2015)
ORIGINAL PAPERS
Submitted: 2015-04-06
Accepted: 2015-09-01
Published online: 2015-09-04

Abstract

Introduction. Although the human umbilical cord (UC) has been previously considered a medical waste, its use as a main source of fetal stem cells for regenerative medicine applications has increased over the past few years. The aim of the study was to assess the impact of the maternal age on the expression of mesenchymal stem cells (MSC) markers CD105 and CD29 in the different areas of human UC.

Material and methods. In this comparative cross sectional study, one hundred term UCs from five maternal age groups (20–45 years) were collected after delivery from healthy mothers and were processed to assess both immuno- and gene expression of CD105 and CD29 surface antigen markers using immunohistochemical and RT-PCR techniques.

Results. The immunoexpression of CD105 and CD29 in the amniotic membrane (AM) and Wharton’s jelly (WJ), the umbilical artery (UA) and the umbilical vein (UV) showed significant negative correlation with the maternal age (p < 0.001). Reduced amount of cells as well as the studied MSC markers and their gene expression levels were documented in older age mothers. CD105-positive MSCs were more abundant in the UA, whereas CD29-positive MSCs were more abundant in the AM and WJ.

Conclusion. The decreased expression of CD105 and CD29 MSCs markers with age suggests that selective isolation of MSCs from Wharton’s jelly, umbilical artery or umbilical vein of younger mothers should be recommended.

Abstract

Introduction. Although the human umbilical cord (UC) has been previously considered a medical waste, its use as a main source of fetal stem cells for regenerative medicine applications has increased over the past few years. The aim of the study was to assess the impact of the maternal age on the expression of mesenchymal stem cells (MSC) markers CD105 and CD29 in the different areas of human UC.

Material and methods. In this comparative cross sectional study, one hundred term UCs from five maternal age groups (20–45 years) were collected after delivery from healthy mothers and were processed to assess both immuno- and gene expression of CD105 and CD29 surface antigen markers using immunohistochemical and RT-PCR techniques.

Results. The immunoexpression of CD105 and CD29 in the amniotic membrane (AM) and Wharton’s jelly (WJ), the umbilical artery (UA) and the umbilical vein (UV) showed significant negative correlation with the maternal age (p < 0.001). Reduced amount of cells as well as the studied MSC markers and their gene expression levels were documented in older age mothers. CD105-positive MSCs were more abundant in the UA, whereas CD29-positive MSCs were more abundant in the AM and WJ.

Conclusion. The decreased expression of CD105 and CD29 MSCs markers with age suggests that selective isolation of MSCs from Wharton’s jelly, umbilical artery or umbilical vein of younger mothers should be recommended.

Get Citation

Keywords

mothers’ age; umbilical cord; MSC; CD29; CD105; RT-PCR; IHC

About this article
Title

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 53, No 3 (2015)

Article type

Original paper

Pages

259-271

Published online

2015-09-04

DOI

10.5603/FHC.a2015.0022

Pubmed

26339985

Bibliographic record

Folia Histochem Cytobiol 2015;53(3):259-271.

Keywords

mothers’ age
umbilical cord
MSC
CD29
CD105
RT-PCR
IHC

Authors

Ghadeer I. Alrefaei
Nasra N. Ayuob
Soad S. Ali
Saleh Al-Karim

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