Vol 49, No 1 (2011)
Original paper
Published online: 2011-04-19
Microvascular abnormalities in capillaroscopy correlate with higher serum IL-18 and sE-selectin levels in patients with type 1 diabetes complicated by microangiopathy
DOI: 10.5603/FHC.2011.0015
Folia Histochem Cytobiol 2011;49(1):104-110.
Abstract
Microvascular abnormalities are one of the most important causes of persistent diabetic complications. The
aim of this study was to compare microvascular changes examined by nailfold capillaroscopy with serum concentrations
of soluble E-selectin (sE-selectin) and IL-18 in type 1 diabetic patients with and without microangiopathy.
Serum levels of sE-selectin and IL-18 were determined by an enzyme-linked immunosorbent assay in 106 patients
with type 1 diabetes and in 40 healthy controls. All diabetic patients were evaluated by extensive clinical, laboratory
and capillaroscopic studies. Morphological changes were observed by nailfold capillaroscopy in 86 out of 106 (81%)
diabetic patients. Severe capillaroscopic changes were seen in 32 out of 54 (59%) patients with microangiopathy, but
in only seven out of 52 (13%) patients without microangiopathy. Higher serum levels of sE-selectin (p < 0.001) and
IL-18 (p < 0.05) were demonstrated in diabetic patients compared to controls. Significant differences of sE-selectin
(p < 0.001) and IL-18 (p < 0.01) serum concentrations were observed between diabetic patients with microangiopathy
and controls. Moreover, comparison between patients with and without microangiopathic complications showed
a significantly higher capillaroscopic score and sE-selectin serum concentration in the group with microangiopathy
(p < 0.001). Furthermore, diabetic patients with severe microvascular changes in capillaroscopy showed significantly
higher IL-18 (p < 0.001) and sE-selectin (p < 0.05) serum levels than subgroups without changes or with mild
abnormalities. Our findings suggest that abnormalities in nailfold capillaroscopy may reflect the extent of microvascular
involvement and are associated with higher sE-selectin and IL-18 serum levels, as well as with microangiopathic
complications in diabetic patients. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 104–110)
Keywords: capillaroscopyIL-18soluble E-selectintype 1 diabetesmicroangiopathy
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