open access

Vol 49, No 1 (2011)
Original paper
Published online: 2011-04-19
Submitted: 2011-12-19
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The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

Katarzyna Łętowska-Andrzejewicz, Anna Torres, Kamil Torres, Piotr Dobrowolski, Tomasz Piersiak, Ryszard Maciejewski, Antoni Gawron, Grzegorz Staśkiewicz, Zbigniew Plewa
DOI: 10.5603/FHC.2011.0012
·
Folia Histochem Cytobiol 2011;49(1):80-89.

open access

Vol 49, No 1 (2011)
ORIGINAL PAPERS
Published online: 2011-04-19
Submitted: 2011-12-19

Abstract

Adjuvant chemotherapy and steroid therapy have been demonstrated to interfere with the wound healing process. The aim of this study was to evaluate the effects of 5-fluorouracil, interferon, and dexamethasone, on the healing of colon anastomosis by assessing morphometric and fractal parameters of the colonic wall. An experimental anastomosis of the ascending colon was performed in 60 male Wistar rats, which were then randomly assigned to four groups. On the second to sixth post-operative days, the rats were administered 5-fluorouracil, interferon-α, dexamethasone, or 0.9% NaCl solution as a control. Macroscopic, histomorphometric and microbiological evaluation was performed in order to assess healing of the anastomosis. In three animals from the dexamethasone group, there was leakage of anastomosis; adhesion formation was highest in the interferon group, and significantly higher than in the control and 5-fluorouracil groups. Histomorphometric parameter alterations were most pronounced on the seventh and fourteenth post-operative days in all treatment groups, with submucosal thickness the most affected parameter. Connective tissue fractal dimension was significantly decreased in those animals treated with interferon and dexamethasone. All three pharmaceutical agents impaired healing of anastomosis, and promoted infection in the anastomosis and skin wound sites. As dexamethasone induced both morphometric and macroscopic alterations, it was considered the most detrimental in this study. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 80–89)

Abstract

Adjuvant chemotherapy and steroid therapy have been demonstrated to interfere with the wound healing process. The aim of this study was to evaluate the effects of 5-fluorouracil, interferon, and dexamethasone, on the healing of colon anastomosis by assessing morphometric and fractal parameters of the colonic wall. An experimental anastomosis of the ascending colon was performed in 60 male Wistar rats, which were then randomly assigned to four groups. On the second to sixth post-operative days, the rats were administered 5-fluorouracil, interferon-α, dexamethasone, or 0.9% NaCl solution as a control. Macroscopic, histomorphometric and microbiological evaluation was performed in order to assess healing of the anastomosis. In three animals from the dexamethasone group, there was leakage of anastomosis; adhesion formation was highest in the interferon group, and significantly higher than in the control and 5-fluorouracil groups. Histomorphometric parameter alterations were most pronounced on the seventh and fourteenth post-operative days in all treatment groups, with submucosal thickness the most affected parameter. Connective tissue fractal dimension was significantly decreased in those animals treated with interferon and dexamethasone. All three pharmaceutical agents impaired healing of anastomosis, and promoted infection in the anastomosis and skin wound sites. As dexamethasone induced both morphometric and macroscopic alterations, it was considered the most detrimental in this study. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 80–89)
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Keywords

colon; anastomosis; healing; 5-fluorouracil; interferon; dexamethasone

About this article
Title

The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 1 (2011)

Article type

Original paper

Pages

80-89

Published online

2011-04-19

DOI

10.5603/FHC.2011.0012

Bibliographic record

Folia Histochem Cytobiol 2011;49(1):80-89.

Keywords

colon
anastomosis
healing
5-fluorouracil
interferon
dexamethasone

Authors

Katarzyna Łętowska-Andrzejewicz
Anna Torres
Kamil Torres
Piotr Dobrowolski
Tomasz Piersiak
Ryszard Maciejewski
Antoni Gawron
Grzegorz Staśkiewicz
Zbigniew Plewa

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