Vol 49, No 1 (2011)
Original paper
Published online: 2011-04-19
Expression and localization of nerve growth factor (NGF) in the testis of alpaca (llama pacos)
DOI: 10.5603/FHC.2011.0009
Folia Histochem Cytobiol 2011;49(1):55-61.
Abstract
During alpaca testis development and spermatogenesis, nerve growth factor (NGF) may play an important
role. The main aim of this study was to determine the expression and localization of NGF in the alpaca
testis, and to discuss the important role of NGF in alpaca reproductive characteristics. Immunohistochemical
staining technique and real-time PCR were used. The expression of NGF in the same cells one-month old
(newborn) alpacas 12-month, and 24-month old alpacas showed significant differences (p < 0.05); 12- and
24-month old alpacas showed no significant differences (p > 0.05); NGF at different cell stages showed no
significant differences (p > 0.05). It suggests that NGF may be involved in the regulation of spermatogenesis,
which provides direct evidence for NGF action in the alpaca testis during postnatal development and spermatogenesis.
(Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 55–61)
Keywords: alpacatestisNGF (nerve growth factor)SABC
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