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Vol 49, No 2 (2011)
Original paper
Submitted: 2011-12-19
Published online: 2011-07-11
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Expression of the transcription factor regulatory factor X1 in the mouse brain

Chenzhuo Feng, Jiejie Li, Zhiyi Zuo
DOI: 10.5603/FHC.2011.0047
·
Folia Histochem Cytobiol 2011;49(2):344-351.

open access

Vol 49, No 2 (2011)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-07-11

Abstract

Limited information indicates that the regulatory factor X1 (RFX1), the prototype member of the transcription factor RFX family, may play a role in the central nervous system. Our recent study showed that knockout of the Rfx1 gene led to early embryonic death. In the present study, we showed that heterozygous Rfx1+/– mice were fertile and grew normally. An abundant amount of RFX1 proteins were expressed in the olfactory bulb, hippocampus and cerebral cortex as detected by b-galactosidase staining (the gene knockout vector contained a coding region for b-galactosidase) and immunofluorescent staining with an anti-RFX1 antibody. RFX1 positive immunostaining was mainly in the nuclei of neurons and microglial cells and was absent from the astrocytes of mouse brains. The heterozygous Rfx1+/– mice expressed RFX1 mRNA and proteins at a level similar to that in the wild-type mice in the olfactory bulb and hippocampus. The expression level of RFX1 proteins was similar in the brains of mice ranging from 15 day old embryos to four month old adults. Our results suggest a significant expression of RFX1 proteins in the mammalian brain. This expression is cell-type and brain-region specific and may take a random monoallelic expression pattern. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 344–351)

Abstract

Limited information indicates that the regulatory factor X1 (RFX1), the prototype member of the transcription factor RFX family, may play a role in the central nervous system. Our recent study showed that knockout of the Rfx1 gene led to early embryonic death. In the present study, we showed that heterozygous Rfx1+/– mice were fertile and grew normally. An abundant amount of RFX1 proteins were expressed in the olfactory bulb, hippocampus and cerebral cortex as detected by b-galactosidase staining (the gene knockout vector contained a coding region for b-galactosidase) and immunofluorescent staining with an anti-RFX1 antibody. RFX1 positive immunostaining was mainly in the nuclei of neurons and microglial cells and was absent from the astrocytes of mouse brains. The heterozygous Rfx1+/– mice expressed RFX1 mRNA and proteins at a level similar to that in the wild-type mice in the olfactory bulb and hippocampus. The expression level of RFX1 proteins was similar in the brains of mice ranging from 15 day old embryos to four month old adults. Our results suggest a significant expression of RFX1 proteins in the mammalian brain. This expression is cell-type and brain-region specific and may take a random monoallelic expression pattern. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 344–351)

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Keywords

brain; microglial cells; mouse; neurons; regulatory factor X1

About this article
Title

Expression of the transcription factor regulatory factor X1 in the mouse brain

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 2 (2011)

Article type

Original paper

Pages

344-351

Published online

2011-07-11

Page views

2710

Article views/downloads

1904

DOI

10.5603/FHC.2011.0047

Bibliographic record

Folia Histochem Cytobiol 2011;49(2):344-351.

Keywords

brain
microglial cells
mouse
neurons
regulatory factor X1

Authors

Chenzhuo Feng
Jiejie Li
Zhiyi Zuo

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