open access

Vol 49, No 2 (2011)
Original paper
Published online: 2011-07-11
Submitted: 2011-12-19
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Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis

Joanna Matowicka-Karna, Maciej Kralisz, Halina Kemona
DOI: 10.5603/FHC.2011.0039
·
Folia Histochem Cytobiol 2011;49(2):280-284.

open access

Vol 49, No 2 (2011)
ORIGINAL PAPERS
Published online: 2011-07-11
Submitted: 2011-12-19

Abstract

The current study aims to determine the involvement of cellular responses in combating Giardia intestinalis invasion. The study group consisted of 44 women and 18 men, aged 18–72 years, infected with G. intestinalis. The diagnosis was established based on laboratory investigations (examination of stool, choloscopy, GSA-65). Blood for analysis was collected before antiparasitic treatment and two weeks after treatment termination. The control group consisted of 22 women and 18 men aged 20–45 years. The serum concentrations of IL-5, IL-6, IL-13, TNF, IFN-γ were assayed using a set of Quantikine human. The concentrations of NO in the serum were determined using a set of Total Nitric Oxide Assay. Patients infected with G. intestinalis showed a statistically significant increase in the levels of NO, IFN-g and IL-13. Even the antiparasitic treatment applied did not reduce the levels of these parameters and only caused a rise in IL-6. Our study showed a lack of acute inflammatory state in the course of G. intestinalis infection. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 280–284)

Abstract

The current study aims to determine the involvement of cellular responses in combating Giardia intestinalis invasion. The study group consisted of 44 women and 18 men, aged 18–72 years, infected with G. intestinalis. The diagnosis was established based on laboratory investigations (examination of stool, choloscopy, GSA-65). Blood for analysis was collected before antiparasitic treatment and two weeks after treatment termination. The control group consisted of 22 women and 18 men aged 20–45 years. The serum concentrations of IL-5, IL-6, IL-13, TNF, IFN-γ were assayed using a set of Quantikine human. The concentrations of NO in the serum were determined using a set of Total Nitric Oxide Assay. Patients infected with G. intestinalis showed a statistically significant increase in the levels of NO, IFN-g and IL-13. Even the antiparasitic treatment applied did not reduce the levels of these parameters and only caused a rise in IL-6. Our study showed a lack of acute inflammatory state in the course of G. intestinalis infection. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 280–284)
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Keywords

giardiosis; IL-5; IL-6; IL-13; TNF-alpha; IFN-gamma; NO

About this article
Title

Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 2 (2011)

Article type

Original paper

Pages

280-284

Published online

2011-07-11

DOI

10.5603/FHC.2011.0039

Bibliographic record

Folia Histochem Cytobiol 2011;49(2):280-284.

Keywords

giardiosis
IL-5
IL-6
IL-13
TNF-alpha
IFN-gamma
NO

Authors

Joanna Matowicka-Karna
Maciej Kralisz
Halina Kemona

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