open access

Vol 52, No 4 (2014)
Review paper
Submitted: 2014-12-17
Accepted: 2014-12-19
Published online: 2014-12-19
Get Citation

Cell-penetrating peptides as a promising tool for delivery of various molecules into the cells

Jaroslaw Ruczynski, Piotr M. Wierzbicki, Marzena Kogut-Wierzbicka, Piotr Mucha, Kamila Siedlecka-Kroplewska, Piotr Rekowski
DOI: 10.5603/FHC.a2014.0034
·
Pubmed: 25530464
·
Folia Histochem Cytobiol 2014;52(4):257-269.

open access

Vol 52, No 4 (2014)
REVIEW
Submitted: 2014-12-17
Accepted: 2014-12-19
Published online: 2014-12-19

Abstract

Many biologically active compounds, including macromolecules that are used as various kinds of drugs, must be delivered to the interior of cell or organelles such as mitochondria or nuclei to achieve a therapeutic effect. However, very often, lipophilic cell membrane is impermeable for these molecules. A new method in the transport of macromolecules through the cell membrane is the one based on utilizing cell-penetrating peptides (CPPs). Invented 25 years ago, CPPs are currently the subject of intensive research in many laboratories all over the world. CPPs are short compounds comprising up to 30 amino acid residues, which penetrate the cell membrane but do not cause cell damage. Additionally, CPPs can transfer hydrophilic molecules (peptides, proteins, nucleic acids) which exceed their mass, and for which the cell membrane is generally impermeable. In this review, we concentrate on the cellular uptake mechanism of CPPs and a method of conjunction of CPPs to the transported molecules. We also highlight the potential of CPPs in delivering various kinds of macromolecules into cells, including compounds of therapeutic interest.

Abstract

Many biologically active compounds, including macromolecules that are used as various kinds of drugs, must be delivered to the interior of cell or organelles such as mitochondria or nuclei to achieve a therapeutic effect. However, very often, lipophilic cell membrane is impermeable for these molecules. A new method in the transport of macromolecules through the cell membrane is the one based on utilizing cell-penetrating peptides (CPPs). Invented 25 years ago, CPPs are currently the subject of intensive research in many laboratories all over the world. CPPs are short compounds comprising up to 30 amino acid residues, which penetrate the cell membrane but do not cause cell damage. Additionally, CPPs can transfer hydrophilic molecules (peptides, proteins, nucleic acids) which exceed their mass, and for which the cell membrane is generally impermeable. In this review, we concentrate on the cellular uptake mechanism of CPPs and a method of conjunction of CPPs to the transported molecules. We also highlight the potential of CPPs in delivering various kinds of macromolecules into cells, including compounds of therapeutic interest.
Get Citation

Keywords

cell-penetrating peptides; membrane transduction peptides; covalent and noncovalent delivery strategy; endocytosis; anti-cancer drug delivery

About this article
Title

Cell-penetrating peptides as a promising tool for delivery of various molecules into the cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 4 (2014)

Article type

Review paper

Pages

257-269

Published online

2014-12-19

DOI

10.5603/FHC.a2014.0034

Pubmed

25530464

Bibliographic record

Folia Histochem Cytobiol 2014;52(4):257-269.

Keywords

cell-penetrating peptides
membrane transduction peptides
covalent and noncovalent delivery strategy
endocytosis
anti-cancer drug delivery

Authors

Jaroslaw Ruczynski
Piotr M. Wierzbicki
Marzena Kogut-Wierzbicka
Piotr Mucha
Kamila Siedlecka-Kroplewska
Piotr Rekowski

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