open access

Vol 52, No 4 (2014)
Original paper
Submitted: 2014-06-04
Accepted: 2014-11-14
Published online: 2014-11-18
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Expression of vascular endothelial growth factor and transforming growth factor alpha in rat testis during chronic renal failure

Yasemin Seval-Celik, Gokhan Akkoyunlu, Erdogan Kocamaz, Ismail Turker Köksal, Sibel Özer, Mehmet Baykara, Ramazan Demir
DOI: 10.5603/FHC.a2014.0032
·
Pubmed: 25410537
·
Folia Histochem Cytobiol 2014;52(4):308-316.

open access

Vol 52, No 4 (2014)
ORIGINAL PAPERS
Submitted: 2014-06-04
Accepted: 2014-11-14
Published online: 2014-11-18

Abstract

Introduction. Vascular endothelial growth factor (VEGF) is known to influence testis function. Transforming growth factor alpha (TGF-α) is expressed in the postnatal testis, and has been demonstrated to stimulate testis development. Systemic diseases such as chronic renal failure (CRF) interfere with hypothalamic-pituitary-go­nadal axis, which may cause defective steroidogenesis and gonadal functions. The aim of this study was to inve­stigate the expression and localization of VEGF and TGF-α in testicular tissues of experimental CRF model. Material and methods. Experimental CRF was induced in rats by the resection of more than 85% of renal mass. The expression of VEGF and TGF-α in testicular tissues were assessed by immunohistochemistry on paraffin sections of control, CRF-nondialysed and CRF-dialysed rats.

Results. The microscopic evaluation of the testicular structure showed that CRF did not affect testicular histology. Immunohistochemical evaluation showed that VEGF was expressed in the cytoplasm of primary and secondary spermatocyte series as well as the early spermatids. Staining intensity was lower in sperma­tocytes going through the first meiotic division. TGF-α was expressed in the nuclei of spermatogonia and primary spermatocytes with stronger staining intensity in spermatogonia. The intensity of VEGF staining was similar in control and experimental animals, however, TGF-α expression was lower in the CRF group.Conclusions. The continuous expression of VEGF in spermatocytes and spermatids suggests that the applied model of CRF does not directly disrupt morphology of seminiferous epithelium, thus also spermiogenesis. However, difference between control rats and CRF group in TGF-α immunopositivity, which was localised in spermatogonial mitosis step, may suggest the interference of CRF with early stages of spermatogenesis.

Abstract

Introduction. Vascular endothelial growth factor (VEGF) is known to influence testis function. Transforming growth factor alpha (TGF-α) is expressed in the postnatal testis, and has been demonstrated to stimulate testis development. Systemic diseases such as chronic renal failure (CRF) interfere with hypothalamic-pituitary-go­nadal axis, which may cause defective steroidogenesis and gonadal functions. The aim of this study was to inve­stigate the expression and localization of VEGF and TGF-α in testicular tissues of experimental CRF model. Material and methods. Experimental CRF was induced in rats by the resection of more than 85% of renal mass. The expression of VEGF and TGF-α in testicular tissues were assessed by immunohistochemistry on paraffin sections of control, CRF-nondialysed and CRF-dialysed rats.

Results. The microscopic evaluation of the testicular structure showed that CRF did not affect testicular histology. Immunohistochemical evaluation showed that VEGF was expressed in the cytoplasm of primary and secondary spermatocyte series as well as the early spermatids. Staining intensity was lower in sperma­tocytes going through the first meiotic division. TGF-α was expressed in the nuclei of spermatogonia and primary spermatocytes with stronger staining intensity in spermatogonia. The intensity of VEGF staining was similar in control and experimental animals, however, TGF-α expression was lower in the CRF group.Conclusions. The continuous expression of VEGF in spermatocytes and spermatids suggests that the applied model of CRF does not directly disrupt morphology of seminiferous epithelium, thus also spermiogenesis. However, difference between control rats and CRF group in TGF-α immunopositivity, which was localised in spermatogonial mitosis step, may suggest the interference of CRF with early stages of spermatogenesis.

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Keywords

chronic renal failure; peritoneal dialysis; rat; testis; spermatogenesis; TGF-α; VEGF; IHC

About this article
Title

Expression of vascular endothelial growth factor and transforming growth factor alpha in rat testis during chronic renal failure

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 4 (2014)

Article type

Original paper

Pages

308-316

Published online

2014-11-18

DOI

10.5603/FHC.a2014.0032

Pubmed

25410537

Bibliographic record

Folia Histochem Cytobiol 2014;52(4):308-316.

Keywords

chronic renal failure
peritoneal dialysis
rat
testis
spermatogenesis
TGF-α
VEGF
IHC

Authors

Yasemin Seval-Celik
Gokhan Akkoyunlu
Erdogan Kocamaz
Ismail Turker Köksal
Sibel Özer
Mehmet Baykara
Ramazan Demir

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