Vol 52, No 3 (2014)
Original paper
Published online: 2014-10-10

open access

Page views 1917
Article views/downloads 1900
Get Citation

Connect on Social Media

Connect on Social Media

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

Fulei Wei, Chenlong Wang, Zhaoxi Wang, Yawei Kong, Yuan Shi, Jianquan Shi, Delin Qi, Hongfang Qi, Jian Liang, Rongqing Zhang, Changzhong Li
DOI: 10.5603/FHC.2014.0027
Folia Histochem Cytobiol 2014;52(3):232-243.

Abstract

The naked carp (Gymnocypris przewalskii), a native teleost, plays an important role in maintenance of the ecological balance in the system of Lake Qinghai (altitude, 3.2 km) on the Qinghai-Tibet Plateau in China. Calcineurin (CN) is the only member of the serine/threonine phosphatase family that can be activated by both Ca2+ and calmodulin (CaM) and involved in many important physiological processes such as salt tolerance/adaption. In this report, cDNAs of CN catalytic subunit paralogue isoforms: GpCAα (GenBank accession no.JQ407043), GpCAγ (GenBank accession no. JQ407043), and CN regulatory subunit (GpCB) (GenBank accession no. JQ410473), were isolated from Gymnocypris przewalskii and their expression patterns in embryos developmentwere characterized. Gene expression profile demonstrated that GpCA and GpCB mRNA was distributed ubiquitously in all embryonic stages and showed decline until final stage of development. Immunohistologicalanalysis revealed CN localization in different tissues including kidney, heart, brain, spermary, and gill. Collectively, these results provide molecular basis and clues to further understand the role of CN during embryos development and its function in tissues for the adaptation mechanism of naked carp.

Article available in PDF format

View PDF Download PDF file