open access

Vol 51, No 4 (2013)
Original paper
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05
Get Citation

Effect of vitamin E and selenium against aluminum-induced nephrotoxicity in pregnant rats

Ghada A. Abdel-Hamid
DOI: 10.5603/FHC.2013.0042
·
Folia Histochem Cytobiol 2013;51(4):312-319.

open access

Vol 51, No 4 (2013)
ORIGINAL PAPERS
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05

Abstract

Abstract: Kidney is one of the most affected organs by aluminium toxicity. This study aimed to investigate the effect of aluminium chloride on the kidney of pregnant rats and to assess the efficiency of vitamin E and selenium in ameliorating this effect. Forty virgin albino rats were randomly divided into two main groups. Control rats were further divided into negative control group (C1, n = 10) which received distilled water and positive control group (C2, n = 10) that received vitamin E (VE, 150 mg/kg/day) and selenium (NaSe 150 μg/kg/day) for 3 months through intra-gastric tube. The experimental group was divided into an E1 subgroup in which rats received aluminium chloride (AlCl3, 150 mg/kg/day, n = 10) and E2 subgroup (n = 10) in which animals received the same dose of AlCl3 plus VE and selenium at the same doses as C2 group for 3 months through intra-gastric tube. Conception of rats was allowed. AlCl3, VE and NaSe were given through intragastric tube during the whole length of the pregnancy, at the same doses as before pregnancy. At the 20th day of gestation dams were sacrificed, kidneys were dissected and processed for routine histological and immunohistochemical staining for identification of T-lymphocytes and macrophages. Integrated optical density of both cell types was assessed. AlCl3 administration induced histopathological changes in the kidney of pregnant rats and increased the density of CD3 and CD68 immunoreactive cells, suggestive of the associated aluminium-induced inflammatory process. Vitamin E and selenium minimized these harmful effects. The results suggest that diets rich in vitamin E and selenium and their supplements are advised particularly during pregnancy to alleviate the effects of possible excessive aluminium exposure.

Abstract

Abstract: Kidney is one of the most affected organs by aluminium toxicity. This study aimed to investigate the effect of aluminium chloride on the kidney of pregnant rats and to assess the efficiency of vitamin E and selenium in ameliorating this effect. Forty virgin albino rats were randomly divided into two main groups. Control rats were further divided into negative control group (C1, n = 10) which received distilled water and positive control group (C2, n = 10) that received vitamin E (VE, 150 mg/kg/day) and selenium (NaSe 150 μg/kg/day) for 3 months through intra-gastric tube. The experimental group was divided into an E1 subgroup in which rats received aluminium chloride (AlCl3, 150 mg/kg/day, n = 10) and E2 subgroup (n = 10) in which animals received the same dose of AlCl3 plus VE and selenium at the same doses as C2 group for 3 months through intra-gastric tube. Conception of rats was allowed. AlCl3, VE and NaSe were given through intragastric tube during the whole length of the pregnancy, at the same doses as before pregnancy. At the 20th day of gestation dams were sacrificed, kidneys were dissected and processed for routine histological and immunohistochemical staining for identification of T-lymphocytes and macrophages. Integrated optical density of both cell types was assessed. AlCl3 administration induced histopathological changes in the kidney of pregnant rats and increased the density of CD3 and CD68 immunoreactive cells, suggestive of the associated aluminium-induced inflammatory process. Vitamin E and selenium minimized these harmful effects. The results suggest that diets rich in vitamin E and selenium and their supplements are advised particularly during pregnancy to alleviate the effects of possible excessive aluminium exposure.
Get Citation

Keywords

aluminium nephrotoxicity; pregnancy; rat; T cells; macrophages; vitamin E; selenium

About this article
Title

Effect of vitamin E and selenium against aluminum-induced nephrotoxicity in pregnant rats

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 4 (2013)

Article type

Original paper

Pages

312-319

Published online

2014-02-05

DOI

10.5603/FHC.2013.0042

Bibliographic record

Folia Histochem Cytobiol 2013;51(4):312-319.

Keywords

aluminium nephrotoxicity
pregnancy
rat
T cells
macrophages
vitamin E
selenium

Authors

Ghada A. Abdel-Hamid

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl