open access

Vol 51, No 4 (2013)
Original paper
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05
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Characterization of common marmoset (Callithrix jacchus) bone marrow-derived mesenchymal stem cells

Akifumi Kanda, Yusuke Sotomaru, Asako Nobukiyo, Emi Yamaoka, Eiso Hiyama
DOI: 10.5603/FHC.2013.0040
·
Folia Histochem Cytobiol 2013;51(4):292-299.

open access

Vol 51, No 4 (2013)
ORIGINAL PAPERS
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05

Abstract

Abstract: Mesenchymal stem cells (MSCs) could be useful for regenerative medicine because they can beharvested easily from the bone marrow of living donors and the cells can be differentiated into adipogenic, osteogenic, and chondrogenic lineages in vitro. To apply MSCs for the medical treatment of human diseases as regenerative medicine, detailed experimental characterization of the cells is required. Recently, a New World primate, the common marmoset (Callithrix jacchus), has been widely used as a new human disease model because of its ease of handling and breeding. Although common marmoset MSCs have been established and will be used in preclinical studies of regenerative medicine, the characteristics of these cells remain unclear. Aiming to characterize common marmoset MSCs further, we harvested common marmoset bone marrow-derived cells (cmBMDCs) from the femurs of newborn males. We revealed that the morphology of the cells was similar to common marmoset fibroblasts, and extracellular matrix components, such as gelatin and fibronectin, were effective for their proliferation and formation of colony-forming unit fibroblasts. Furthermore, we were able to differentiate cmBMDCs into adipocytes, osteocytes, and chondrocytes in vitro, and they expressed the MSCmarkers CD44, CD73, CD90, and CD105, but their expression decreased with increasing passage number. The data demonstrate that cmBMDCs exhibit characteristics of MSCs and thus it would be beneficial to use these cells in preclinical studies.

Abstract

Abstract: Mesenchymal stem cells (MSCs) could be useful for regenerative medicine because they can beharvested easily from the bone marrow of living donors and the cells can be differentiated into adipogenic, osteogenic, and chondrogenic lineages in vitro. To apply MSCs for the medical treatment of human diseases as regenerative medicine, detailed experimental characterization of the cells is required. Recently, a New World primate, the common marmoset (Callithrix jacchus), has been widely used as a new human disease model because of its ease of handling and breeding. Although common marmoset MSCs have been established and will be used in preclinical studies of regenerative medicine, the characteristics of these cells remain unclear. Aiming to characterize common marmoset MSCs further, we harvested common marmoset bone marrow-derived cells (cmBMDCs) from the femurs of newborn males. We revealed that the morphology of the cells was similar to common marmoset fibroblasts, and extracellular matrix components, such as gelatin and fibronectin, were effective for their proliferation and formation of colony-forming unit fibroblasts. Furthermore, we were able to differentiate cmBMDCs into adipocytes, osteocytes, and chondrocytes in vitro, and they expressed the MSCmarkers CD44, CD73, CD90, and CD105, but their expression decreased with increasing passage number. The data demonstrate that cmBMDCs exhibit characteristics of MSCs and thus it would be beneficial to use these cells in preclinical studies.
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Keywords

common marmoset; mesenchymal stem cells; extracellular matrix; in vitro differentiation; cell surface proteins

About this article
Title

Characterization of common marmoset (Callithrix jacchus) bone marrow-derived mesenchymal stem cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 4 (2013)

Article type

Original paper

Pages

292-299

Published online

2014-02-05

DOI

10.5603/FHC.2013.0040

Bibliographic record

Folia Histochem Cytobiol 2013;51(4):292-299.

Keywords

common marmoset
mesenchymal stem cells
extracellular matrix
in vitro differentiation
cell surface proteins

Authors

Akifumi Kanda
Yusuke Sotomaru
Asako Nobukiyo
Emi Yamaoka
Eiso Hiyama

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