open access

Vol 52, No 2 (2014)
Original paper
Submitted: 2013-11-02
Accepted: 2014-05-13
Published online: 2014-07-09
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Expression of ENaC, SGK1 and Nedd4 isoforms in the cochlea of guinea pig

Shi-Xun Zhong, Guo-Hua Hu, Zhao-Hua Liu
DOI: 10.5603/FHC.2014.0010
·
Folia Histochem Cytobiol 2014;52(2):144-148.

open access

Vol 52, No 2 (2014)
ORIGINAL PAPERS
Submitted: 2013-11-02
Accepted: 2014-05-13
Published online: 2014-07-09

Abstract

It has been demonstrated that the epithelial sodium channel (ENaC) may play critical roles in (re)absorbing Na+ from apical plasma membrane in various tissues and cells. Moreover, the serum glucocorticoid-inducible kinase 1 (SGK1) and the ubiquitin-protein ligase neural precursor cell-expressed, developmentally downregulated isoforms Nedd4 are involved in ENaC regulation in response to hormones such as aldosterone, vasopressin and insulin. The aim of the study was to investigate the cellular localizations of ENaC subunits, SGK1, and Nedd4 isoforms in the cochlea of guinea pig. Ten adult guinea pigs were sacrificed and their cochleas were collected. The expression patterns of ENaC subunits, SGK1 and Nedd4-2 isoforms in the guinea pig cochlea were studied by immunohistochemistry with the specific polyclonal rabbit antibodies against rat α-, β- and γ-ENaC, SGK1, Nedd4-1/2 and Nedd4-2. The results showed that all these proteins were extensively expressed in various regions of the cochlea. They were found in the spiral ligament, organ of Corti, spiral limbus, spiral ganglion and Reissner’s membrane with different staining patterns. The results indicated that a Na+ transport system may exist in the cochlea of guinea pig consisting of ENaC, SGK1 and Nedd4, which may work in concert to transport Na+ and to maintain homeostasis in inner ear as it does in other epithelia.

Abstract

It has been demonstrated that the epithelial sodium channel (ENaC) may play critical roles in (re)absorbing Na+ from apical plasma membrane in various tissues and cells. Moreover, the serum glucocorticoid-inducible kinase 1 (SGK1) and the ubiquitin-protein ligase neural precursor cell-expressed, developmentally downregulated isoforms Nedd4 are involved in ENaC regulation in response to hormones such as aldosterone, vasopressin and insulin. The aim of the study was to investigate the cellular localizations of ENaC subunits, SGK1, and Nedd4 isoforms in the cochlea of guinea pig. Ten adult guinea pigs were sacrificed and their cochleas were collected. The expression patterns of ENaC subunits, SGK1 and Nedd4-2 isoforms in the guinea pig cochlea were studied by immunohistochemistry with the specific polyclonal rabbit antibodies against rat α-, β- and γ-ENaC, SGK1, Nedd4-1/2 and Nedd4-2. The results showed that all these proteins were extensively expressed in various regions of the cochlea. They were found in the spiral ligament, organ of Corti, spiral limbus, spiral ganglion and Reissner’s membrane with different staining patterns. The results indicated that a Na+ transport system may exist in the cochlea of guinea pig consisting of ENaC, SGK1 and Nedd4, which may work in concert to transport Na+ and to maintain homeostasis in inner ear as it does in other epithelia.
Get Citation

Keywords

guinea pig; cochlea; ENaC; SGK1; Nedd4 isoforms; immunohistochemistry

About this article
Title

Expression of ENaC, SGK1 and Nedd4 isoforms in the cochlea of guinea pig

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 2 (2014)

Article type

Original paper

Pages

144-148

Published online

2014-07-09

DOI

10.5603/FHC.2014.0010

Bibliographic record

Folia Histochem Cytobiol 2014;52(2):144-148.

Keywords

guinea pig
cochlea
ENaC
SGK1
Nedd4 isoforms
immunohistochemistry

Authors

Shi-Xun Zhong
Guo-Hua Hu
Zhao-Hua Liu

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