open access

Vol 52, No 1 (2014)
Original paper
Submitted: 2013-07-15
Accepted: 2014-03-07
Published online: 2014-05-07
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Effect of apelin on mitosis, apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2

Hanna Antushevich, Agata Krawczynska, Malgorzata Kapica, Andrzej Przemyslaw Herman, Romuald Zabielski
DOI: 10.5603/FHC.2014.0006
·
Folia Histochem Cytobiol 2014;52(1):51-59.

open access

Vol 52, No 1 (2014)
ORIGINAL PAPERS
Submitted: 2013-07-15
Accepted: 2014-03-07
Published online: 2014-05-07

Abstract

Apelin is a regulatory peptide, identified as an endogenous ligand of the Apelin receptor (APJ). Both the apelin and the APJ were detected in brain, lung, heart, mammary gland, kidney, placenta, adipose tissues and the gastrointestinal tract. Apelin is considered an important regulatory gut peptide with a potential physiological role in gastrointestinal cytoprotection, regulation of food intake and drinking behaviour. The aim of the present study was to assess the effect of the apelin on mitosis, apoptosis and the expression of DNA repair enzyme (OGG 1/2), and APJ receptor in intestinal cell lines: rat crypt (IEC-6) and human enterocyte model (Caco-2). The cell cultures were incubated with the apelin-12 (10–8 M) for 4, 6, 12, 24 and 48 h and the apoptosis (caspase 3), mitosis (Ki-67) and DNA repair enzyme (OGG1/2) markers were studied by Real-Time qRT-PCR and immunofluorescent methods. The results of Real-Time qRT-PCR and immunocytochemical analysis showed that the levels of mRNAs were inversely related to the expression level of corresponding proteins. Immunofluorescent studies revealed inhibitory effect of apelin-12 on apoptosis, mitosis and the expression of OGG1/2 in the intestinal crypt cell line IEC-6. However, in the enterocyte model Caco-2 cells apelin stimulated apoptosis and mitosis, and reduced OGG1/2 expression. These findings suggest that apelin may be involved in the control of epithelial cell turnover in the gastrointestinal tract. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 51–59)

Abstract

Apelin is a regulatory peptide, identified as an endogenous ligand of the Apelin receptor (APJ). Both the apelin and the APJ were detected in brain, lung, heart, mammary gland, kidney, placenta, adipose tissues and the gastrointestinal tract. Apelin is considered an important regulatory gut peptide with a potential physiological role in gastrointestinal cytoprotection, regulation of food intake and drinking behaviour. The aim of the present study was to assess the effect of the apelin on mitosis, apoptosis and the expression of DNA repair enzyme (OGG 1/2), and APJ receptor in intestinal cell lines: rat crypt (IEC-6) and human enterocyte model (Caco-2). The cell cultures were incubated with the apelin-12 (10–8 M) for 4, 6, 12, 24 and 48 h and the apoptosis (caspase 3), mitosis (Ki-67) and DNA repair enzyme (OGG1/2) markers were studied by Real-Time qRT-PCR and immunofluorescent methods. The results of Real-Time qRT-PCR and immunocytochemical analysis showed that the levels of mRNAs were inversely related to the expression level of corresponding proteins. Immunofluorescent studies revealed inhibitory effect of apelin-12 on apoptosis, mitosis and the expression of OGG1/2 in the intestinal crypt cell line IEC-6. However, in the enterocyte model Caco-2 cells apelin stimulated apoptosis and mitosis, and reduced OGG1/2 expression. These findings suggest that apelin may be involved in the control of epithelial cell turnover in the gastrointestinal tract. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 51–59)

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Keywords

apelin; APJ; caspase 3; Ki-67; OGG 1/2; QPCR; immunofluorescence

About this article
Title

Effect of apelin on mitosis, apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 1 (2014)

Article type

Original paper

Pages

51-59

Published online

2014-05-07

DOI

10.5603/FHC.2014.0006

Bibliographic record

Folia Histochem Cytobiol 2014;52(1):51-59.

Keywords

apelin
APJ
caspase 3
Ki-67
OGG 1/2
QPCR
immunofluorescence

Authors

Hanna Antushevich
Agata Krawczynska
Malgorzata Kapica
Andrzej Przemyslaw Herman
Romuald Zabielski

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