open access

Vol 52, No 1 (2014)
Original paper
Submitted: 2013-07-01
Accepted: 2014-03-07
Published online: 2014-05-07
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A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine

Siham K. Abunasef, Hanan A. Amin, Ghada A. Abdel-Hamid
DOI: 10.5603/FHC.2014.0005
·
Folia Histochem Cytobiol 2014;52(1):42-50.

open access

Vol 52, No 1 (2014)
ORIGINAL PAPERS
Submitted: 2013-07-01
Accepted: 2014-03-07
Published online: 2014-05-07

Abstract

In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 42–50)

Abstract

In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 42–50)

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Keywords

streptozotocin; caffeine; diabetes; insulin; islet structure; beta cells; IHC

About this article
Title

A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 1 (2014)

Article type

Original paper

Pages

42-50

Published online

2014-05-07

DOI

10.5603/FHC.2014.0005

Bibliographic record

Folia Histochem Cytobiol 2014;52(1):42-50.

Keywords

streptozotocin
caffeine
diabetes
insulin
islet structure
beta cells
IHC

Authors

Siham K. Abunasef
Hanan A. Amin
Ghada A. Abdel-Hamid

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