Vol 51, No 3 (2013)
Original paper
Submitted: 2013-07-02
Accepted: 2013-09-16
Published online: 2013-11-07
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Anti-TNF antibodies do not induce the apoptosis of lamina propria mononuclear cells in uninflamed intestinal tissue in patients with Crohn’s disease

Piotr Eder, Liliana Lykowska-Szuber, Iwona Krela-Kazmierczak, Kamila Stawczyk-Eder, Karolina Sterzynska, Katarzyna Iwanik, Przemyslaw Majewski, Maciej Zabel, Krzysztof Linke
DOI: 10.5603/FHC.2013.0034
·
Folia Histochem Cytobiol 2013;51(3):239-243.
Vol 51, No 3 (2013)
ORIGINAL PAPERS
Submitted: 2013-07-02
Accepted: 2013-09-16
Published online: 2013-11-07

Abstract

It is not known if anti-tumor necrosis factor (anti-TNF) agents provoke only apoptosis of lamina propria mononuclear cells (LPMC) engaged in inflammatory processes or whether it’s a general phenomenon concerning all LPMC. In this study we carried out an immunohistochemical analysis of the expression of several apoptosis-related proteins (active caspase-3, Bax, Bcl-2, Fas, TNFR1, CD4, and CD8) in uninflamed mucosa in Crohn’s disease (CD) patients treated with anti-TNF agents. 16 CD patients (mean age 34 ± 11, mean disease duration 7 ± 5 years) were included in the study. 10 patients were treated with infliximab and 6 — with adalimumab. The expression of active caspase 3, Bax, Bcl-2, Fas, TNFR1 and CD8 in LPMC did not change significantly after the therapy. We concluded that anti-TNF antibodies did not promote LPMC apoptosis in uninflamed tissues. This is in contrast to the phenomena observed in inflamed tissues. These data show that anti-TNF antibodies rather restore the susceptibility to apoptosis of LPMC in inflamed areas of the gut in CD, than directly induce LPMC apoptosis; otherwise the anti-TNF antibodies should have also induced apoptosis in the uninflamed mucosa.

Abstract

It is not known if anti-tumor necrosis factor (anti-TNF) agents provoke only apoptosis of lamina propria mononuclear cells (LPMC) engaged in inflammatory processes or whether it’s a general phenomenon concerning all LPMC. In this study we carried out an immunohistochemical analysis of the expression of several apoptosis-related proteins (active caspase-3, Bax, Bcl-2, Fas, TNFR1, CD4, and CD8) in uninflamed mucosa in Crohn’s disease (CD) patients treated with anti-TNF agents. 16 CD patients (mean age 34 ± 11, mean disease duration 7 ± 5 years) were included in the study. 10 patients were treated with infliximab and 6 — with adalimumab. The expression of active caspase 3, Bax, Bcl-2, Fas, TNFR1 and CD8 in LPMC did not change significantly after the therapy. We concluded that anti-TNF antibodies did not promote LPMC apoptosis in uninflamed tissues. This is in contrast to the phenomena observed in inflamed tissues. These data show that anti-TNF antibodies rather restore the susceptibility to apoptosis of LPMC in inflamed areas of the gut in CD, than directly induce LPMC apoptosis; otherwise the anti-TNF antibodies should have also induced apoptosis in the uninflamed mucosa.

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Keywords

apoptosis; Crohn’s disease; infliximab; adalimumab; mononuclear cells; uninflamed mucosa

About this article
Title

Anti-TNF antibodies do not induce the apoptosis of lamina propria mononuclear cells in uninflamed intestinal tissue in patients with Crohn’s disease

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 3 (2013)

Article type

Original paper

Pages

239-243

Published online

2013-11-07

DOI

10.5603/FHC.2013.0034

Bibliographic record

Folia Histochem Cytobiol 2013;51(3):239-243.

Keywords

apoptosis
Crohn’s disease
infliximab
adalimumab
mononuclear cells
uninflamed mucosa

Authors

Piotr Eder
Liliana Lykowska-Szuber
Iwona Krela-Kazmierczak
Kamila Stawczyk-Eder
Karolina Sterzynska
Katarzyna Iwanik
Przemyslaw Majewski
Maciej Zabel
Krzysztof Linke

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