open access

Vol 51, No 1 (2013)
Original paper
Submitted: 2013-04-28
Accepted: 2013-04-28
Published online: 2013-04-24
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Monobutyl phthalate induces the expression change of G-Protein-Coupled Receptor 30 in rat testicular Sertoli cells

Yang Hu, Dongmei Li, Yuqiu Lu, Xiaodong Han
DOI: 10.5603/FHC.2013.003
·
Folia Histochem Cytobiol 2013;51(1):18-24.

open access

Vol 51, No 1 (2013)
ORIGINAL PAPERS
Submitted: 2013-04-28
Accepted: 2013-04-28
Published online: 2013-04-24

Abstract

The aim of the study was to explore whether G-Protein-Coupled Receptor 30 (GPR30) was expressed in rat testicular Sertoli cells and to assess the impact of monobutyl phthalate (MBP) on the expression of GPR30 in Sertoli cells. By using RT-PCR, Western-Blot and immunofluorescent microscopy, the expression of GPR30 in rat Sertoli cells was found at both gene and protein level. Cultures of Sertoli cells were exposed to MBP (10– –1000 mM) or a vehicle. The results indicated that the expression of GPR30 increased at gene and protein levels in Sertoli cells following administration of MBP even at a relatively low concentration. We suggest that changes of GPR30 expression may play an important role in the effects of the xenoestrogen MBP on Sertoli cell function. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 18–24)

Abstract

The aim of the study was to explore whether G-Protein-Coupled Receptor 30 (GPR30) was expressed in rat testicular Sertoli cells and to assess the impact of monobutyl phthalate (MBP) on the expression of GPR30 in Sertoli cells. By using RT-PCR, Western-Blot and immunofluorescent microscopy, the expression of GPR30 in rat Sertoli cells was found at both gene and protein level. Cultures of Sertoli cells were exposed to MBP (10– –1000 mM) or a vehicle. The results indicated that the expression of GPR30 increased at gene and protein levels in Sertoli cells following administration of MBP even at a relatively low concentration. We suggest that changes of GPR30 expression may play an important role in the effects of the xenoestrogen MBP on Sertoli cell function. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 18–24)

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Keywords

monobutyl phthalate; GPR30; Sertoli cell; xenoestrogen; nongenomic action; rat

About this article
Title

Monobutyl phthalate induces the expression change of G-Protein-Coupled Receptor 30 in rat testicular Sertoli cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 1 (2013)

Article type

Original paper

Pages

18-24

Published online

2013-04-24

DOI

10.5603/FHC.2013.003

Bibliographic record

Folia Histochem Cytobiol 2013;51(1):18-24.

Keywords

monobutyl phthalate
GPR30
Sertoli cell
xenoestrogen
nongenomic action
rat

Authors

Yang Hu
Dongmei Li
Yuqiu Lu
Xiaodong Han

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