open access

Vol 51, No 1 (2013)
Original paper
Submitted: 2013-04-28
Accepted: 2013-04-28
Published online: 2013-04-24
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12-O-tetradecanoylphorbol-1,3-acetate-induced degradation of protein kinase B via ubiquitin- -proteasomal pathway depends on its Ser473 phosphorylation in gastric cancer cells

Bing Zhang, Chun Xia
DOI: 10.5603/FHC.2013.002
·
Folia Histochem Cytobiol 2013;51(1):11-17.

open access

Vol 51, No 1 (2013)
ORIGINAL PAPERS
Submitted: 2013-04-28
Accepted: 2013-04-28
Published online: 2013-04-24

Abstract

TPA (12-O-tetradecanoylphorbol-1, 3-acetate) can induce cell apoptosis and cause PKB (protein kinase B) degradation correlated with its phosphorylation in gastric cancer cells. We investigated whether the ubiquitin-proteasomal pathway is involved in TPA-induced PKB degradation. The results showed that TPA could induce PKB ubiquitination by inhibiting its phosphorylation at the serine 473 site. Moreover, MG132 (26S proteasome inhibitor) partially inhibited TPA-induced degradation of PKB. Taken together, TPA could degrade PKB via the ubiquitin-proteasomal pathway, and the suppression of PKB phosphorylation at the serine 473 site might be a prerequisite for the TPA-induced ubiquitination in gastric cancer cells. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 11–17)

Abstract

TPA (12-O-tetradecanoylphorbol-1, 3-acetate) can induce cell apoptosis and cause PKB (protein kinase B) degradation correlated with its phosphorylation in gastric cancer cells. We investigated whether the ubiquitin-proteasomal pathway is involved in TPA-induced PKB degradation. The results showed that TPA could induce PKB ubiquitination by inhibiting its phosphorylation at the serine 473 site. Moreover, MG132 (26S proteasome inhibitor) partially inhibited TPA-induced degradation of PKB. Taken together, TPA could degrade PKB via the ubiquitin-proteasomal pathway, and the suppression of PKB phosphorylation at the serine 473 site might be a prerequisite for the TPA-induced ubiquitination in gastric cancer cells. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 11–17)

Get Citation

Keywords

Akt; PKB; phosphorylation; ubiquitin-proteasomal pathway; gastric cancer cells

About this article
Title

12-O-tetradecanoylphorbol-1,3-acetate-induced degradation of protein kinase B via ubiquitin- -proteasomal pathway depends on its Ser473 phosphorylation in gastric cancer cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 1 (2013)

Article type

Original paper

Pages

11-17

Published online

2013-04-24

DOI

10.5603/FHC.2013.002

Bibliographic record

Folia Histochem Cytobiol 2013;51(1):11-17.

Keywords

Akt
PKB
phosphorylation
ubiquitin-proteasomal pathway
gastric cancer cells

Authors

Bing Zhang
Chun Xia

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