Vol 51, No 1 (2013)
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Published online: 2013-04-24

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12-O-tetradecanoylphorbol-1,3-acetate-induced degradation of protein kinase B via ubiquitin- -proteasomal pathway depends on its Ser473 phosphorylation in gastric cancer cells

Bing Zhang, Chun Xia
DOI: 10.5603/FHC.2013.002
Folia Histochem Cytobiol 2013;51(1):11-17.

Abstract

TPA (12-O-tetradecanoylphorbol-1, 3-acetate) can induce cell apoptosis and cause PKB (protein kinase B) degradation correlated with its phosphorylation in gastric cancer cells. We investigated whether the ubiquitin-proteasomal pathway is involved in TPA-induced PKB degradation. The results showed that TPA could induce PKB ubiquitination by inhibiting its phosphorylation at the serine 473 site. Moreover, MG132 (26S proteasome inhibitor) partially inhibited TPA-induced degradation of PKB. Taken together, TPA could degrade PKB via the ubiquitin-proteasomal pathway, and the suppression of PKB phosphorylation at the serine 473 site might be a prerequisite for the TPA-induced ubiquitination in gastric cancer cells. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 11–17)

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