Vol 51, No 3 (2013)
Original paper
Submitted: 2013-04-16
Accepted: 2013-07-20
Published online: 2013-11-07
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Sonic hedgehog pathway dysregulation in skin basal-cell carcinoma of a Polish population

Aleksandra Lesiak, Dorota Sobolewska-Sztychny, Marian Danilewicz, Michal Rogowski-Tylman, Anna Sysa-Jedrzejowska, Michal Sobjanek, Irmina Olejniczak-Staruch, Joanna Narbutt
DOI: 10.5603/FHC.2013.0031
·
Folia Histochem Cytobiol 2013;51(3):219-224.
Vol 51, No 3 (2013)
ORIGINAL PAPERS
Submitted: 2013-04-16
Accepted: 2013-07-20
Published online: 2013-11-07

Abstract

Sonic hedgehog (Shh) pathway impairment plays a key role in the pathogenesis of basal-cell carcinomas (BCC), the most frequent skin tumor among Caucasians. Shh, Smo, and Gli2 family proteins are necessary for adequate and controlled cell proliferation. The aim of this study was to evaluate Shh, Smo, and Smo expression in BCC skin biopsies taken from sun-exposed areas. 41 BCC skin biopsies and 22 healthy skin specimens (the control group) taken from the same areas served as material for the study. All specimens were immunohistochemically stained with monoclonal antibodies directed against the chosen proteins. Shh and Smo expression (cytoplasmic pattern) were recorded semiquantitatively using a four-grade score (0–3). Gli2 expression (nuclear pattern) was determined using an image analysis system (semiautomatic function). The immunoexpression of the Shh and Smo proteins significantly increased in the BCC group, as compared with the normal controls (for Shh, the mean intensity was 1.67 in BCC vs. 1.17 in the control group, p < 0.001; for Smo, the mean intensity was 1.46 in BCC vs. 0.99 in the control group, p < 0.001). The staining for Gli2 in the BCC group was completely negative, but indicated the presence of Gli2 in the control patients (1.15 Gli2+ cells/100 cells). Sonic hedgehog pathway dysregulation may play an important role in skin cancerogenesis leading to BCC development.

Abstract

Sonic hedgehog (Shh) pathway impairment plays a key role in the pathogenesis of basal-cell carcinomas (BCC), the most frequent skin tumor among Caucasians. Shh, Smo, and Gli2 family proteins are necessary for adequate and controlled cell proliferation. The aim of this study was to evaluate Shh, Smo, and Smo expression in BCC skin biopsies taken from sun-exposed areas. 41 BCC skin biopsies and 22 healthy skin specimens (the control group) taken from the same areas served as material for the study. All specimens were immunohistochemically stained with monoclonal antibodies directed against the chosen proteins. Shh and Smo expression (cytoplasmic pattern) were recorded semiquantitatively using a four-grade score (0–3). Gli2 expression (nuclear pattern) was determined using an image analysis system (semiautomatic function). The immunoexpression of the Shh and Smo proteins significantly increased in the BCC group, as compared with the normal controls (for Shh, the mean intensity was 1.67 in BCC vs. 1.17 in the control group, p < 0.001; for Smo, the mean intensity was 1.46 in BCC vs. 0.99 in the control group, p < 0.001). The staining for Gli2 in the BCC group was completely negative, but indicated the presence of Gli2 in the control patients (1.15 Gli2+ cells/100 cells). Sonic hedgehog pathway dysregulation may play an important role in skin cancerogenesis leading to BCC development.

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Keywords

basal-cell carcinoma; Shh; Smo; Gli2; normal epidermis; ultraviolet radiation

About this article
Title

Sonic hedgehog pathway dysregulation in skin basal-cell carcinoma of a Polish population

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 3 (2013)

Article type

Original paper

Pages

219-224

Published online

2013-11-07

DOI

10.5603/FHC.2013.0031

Bibliographic record

Folia Histochem Cytobiol 2013;51(3):219-224.

Keywords

basal-cell carcinoma
Shh
Smo
Gli2
normal epidermis
ultraviolet radiation

Authors

Aleksandra Lesiak
Dorota Sobolewska-Sztychny
Marian Danilewicz
Michal Rogowski-Tylman
Anna Sysa-Jedrzejowska
Michal Sobjanek
Irmina Olejniczak-Staruch
Joanna Narbutt

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