Vol 51, No 3 (2013)
Original paper
Submitted: 2013-03-11
Accepted: 2013-07-29
Published online: 2013-11-07
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Expression of selected proteins in breast cancer brain metastases

Ondrej Gojis, Martina Kubecova, Jozef Rosina, Jana Vranova, Martin Celko, Denisa Frajerova, Jan Zmrhal, Jozef Zahumensky, Tereza Bacova, Vaclav Baca, Vaclav Mandys, Eduard Kucera
DOI: 10.5603/FHC.2013.0030
·
Folia Histochem Cytobiol 2013;51(3):213-218.
Vol 51, No 3 (2013)
ORIGINAL PAPERS
Submitted: 2013-03-11
Accepted: 2013-07-29
Published online: 2013-11-07

Abstract

The aim of the study was to assess the immunohistochemical (IHC) profiles of SRC3, Pax2, ER, PgR, Her2, EGFR, CK5/6, and Ki67 proteins in breast-cancer brain metastasis. The study utilized tumor samples from 30 metastatic patients and calculated correlations between all IHC variables. In fourteen cases, primary breast cancers paired with secondary deposits were analyzed. We evaluated the association between IHC status in the primary and secondary deposits, grade, and histotype of the tumors. The examination of the metastatic deposits in all 30 patients resulted in positive detection in the following cases: SRC3 in 20 cases (66.6%), Pax2 in 22 (73.3%), ER in 22 (73.3%), PgR in 25 (83.3%), Her2 in 10 (33.3%), EGFR in 12 (40%), CK5/6 in 7 (23.3%), and Ki67 in 23 (76.6%). Grade 2 was found in 13.3% of all patients, and grade 3 in 86.7%. SRC3 and Pax2 were positive in both G2 and G3. Invasive lobular carcinoma and invasive ductal carcinoma were diagnosed in 23.3% and 76.7% of cases, respectively. There were no differences between the IHC expression of the studied proteins in either grading or histotype of the tumors. In the IHC profiles, which included SRC3, Pax2, ER, PgR, Her2, CK5/6, Ki67, and EGFR, we found no statistically significant differences between the primary cancer and the brain metastasis. In our study of metastatic breast carcinoma deposits, there was no correlation between SRC3, Pax2 status and histotype, and tumor grade. The IHC status of the paired primary and metastatic deposits did not differ in a statistically significant manner.

Abstract

The aim of the study was to assess the immunohistochemical (IHC) profiles of SRC3, Pax2, ER, PgR, Her2, EGFR, CK5/6, and Ki67 proteins in breast-cancer brain metastasis. The study utilized tumor samples from 30 metastatic patients and calculated correlations between all IHC variables. In fourteen cases, primary breast cancers paired with secondary deposits were analyzed. We evaluated the association between IHC status in the primary and secondary deposits, grade, and histotype of the tumors. The examination of the metastatic deposits in all 30 patients resulted in positive detection in the following cases: SRC3 in 20 cases (66.6%), Pax2 in 22 (73.3%), ER in 22 (73.3%), PgR in 25 (83.3%), Her2 in 10 (33.3%), EGFR in 12 (40%), CK5/6 in 7 (23.3%), and Ki67 in 23 (76.6%). Grade 2 was found in 13.3% of all patients, and grade 3 in 86.7%. SRC3 and Pax2 were positive in both G2 and G3. Invasive lobular carcinoma and invasive ductal carcinoma were diagnosed in 23.3% and 76.7% of cases, respectively. There were no differences between the IHC expression of the studied proteins in either grading or histotype of the tumors. In the IHC profiles, which included SRC3, Pax2, ER, PgR, Her2, CK5/6, Ki67, and EGFR, we found no statistically significant differences between the primary cancer and the brain metastasis. In our study of metastatic breast carcinoma deposits, there was no correlation between SRC3, Pax2 status and histotype, and tumor grade. The IHC status of the paired primary and metastatic deposits did not differ in a statistically significant manner.

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Keywords

brain metastases; breast cancer; protein expression; SRC3; Pax2; ER; PgR; Her2; CK5/6; Ki67; EGFR

About this article
Title

Expression of selected proteins in breast cancer brain metastases

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 3 (2013)

Article type

Original paper

Pages

213-218

Published online

2013-11-07

DOI

10.5603/FHC.2013.0030

Bibliographic record

Folia Histochem Cytobiol 2013;51(3):213-218.

Keywords

brain metastases
breast cancer
protein expression
SRC3
Pax2
ER
PgR
Her2
CK5/6
Ki67
EGFR

Authors

Ondrej Gojis
Martina Kubecova
Jozef Rosina
Jana Vranova
Martin Celko
Denisa Frajerova
Jan Zmrhal
Jozef Zahumensky
Tereza Bacova
Vaclav Baca
Vaclav Mandys
Eduard Kucera

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