open access

Vol 50, No 4 (2012)
Original paper
Submitted: 2012-12-23
Accepted: 2012-12-23
Published online: 2012-12-23
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Assessment of inflammatory infiltration and angiogenesis in the thrombus and the wall of abdominal aortic aneurysms on the basis of histological parameters and computed tomography angiography study

Adam Lukasiewicz, Joanna Reszec, Radoslaw Kowalewski, Lech Chyczewski, Urszula Lebkowska
DOI: 10.5603/FHC.2012.0077
·
Folia Histochem Cytobiol 2012;50(4):547-553.

open access

Vol 50, No 4 (2012)
ORIGINAL PAPERS
Submitted: 2012-12-23
Accepted: 2012-12-23
Published online: 2012-12-23

Abstract

The proliferation of vessels within the aneurysm’s wall and the intraluminal thrombus of abdominal
aortic aneurysm (AAA) may be the main factor responsible for progression and rupture of AAA. The aim of this
study was to compare the parameters of the thrombus (size, density, contrast enhancement) measured by computed
tomography (CT) with histological assessment of thrombi removed during surgery. 29 patients with AAA
were examined with angio-CT. Post-surgery histopathological evaluation of AAA was performed. Slides were
stained with markers of B- (CD20) and T-lymphocytes (CD3), and markers of endothelial cells (CD34). Thrombi
were enhanced after contrast media administration in angio-CT (p = 0.002). There was a statistically significant
correlation between contrast enhancement and the presence of B lymphocytes. Intensity of endothelial cell
marker expression significantly correlated with the presence of inflammatory T- and B-cells. No statistical significant
correlation was found between contrast enhancement of the thrombus and markers of endothelial cells.
The accumulation of inflammatory cells in the wall of AAA thrombus results in the formation of new vessels
which participates to the instability of the thrombus and AAA wall. Assessment of the inflammation and neovascularization
in the wall and thrombus of the AAA might be an important factor in monitoring the progression
and the risk of aneurysm’s rupture.

Abstract

The proliferation of vessels within the aneurysm’s wall and the intraluminal thrombus of abdominal
aortic aneurysm (AAA) may be the main factor responsible for progression and rupture of AAA. The aim of this
study was to compare the parameters of the thrombus (size, density, contrast enhancement) measured by computed
tomography (CT) with histological assessment of thrombi removed during surgery. 29 patients with AAA
were examined with angio-CT. Post-surgery histopathological evaluation of AAA was performed. Slides were
stained with markers of B- (CD20) and T-lymphocytes (CD3), and markers of endothelial cells (CD34). Thrombi
were enhanced after contrast media administration in angio-CT (p = 0.002). There was a statistically significant
correlation between contrast enhancement and the presence of B lymphocytes. Intensity of endothelial cell
marker expression significantly correlated with the presence of inflammatory T- and B-cells. No statistical significant
correlation was found between contrast enhancement of the thrombus and markers of endothelial cells.
The accumulation of inflammatory cells in the wall of AAA thrombus results in the formation of new vessels
which participates to the instability of the thrombus and AAA wall. Assessment of the inflammation and neovascularization
in the wall and thrombus of the AAA might be an important factor in monitoring the progression
and the risk of aneurysm’s rupture.
Get Citation
About this article
Title

Assessment of inflammatory infiltration and angiogenesis in the thrombus and the wall of abdominal aortic aneurysms on the basis of histological parameters and computed tomography angiography study

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 4 (2012)

Article type

Original paper

Pages

547-553

Published online

2012-12-23

DOI

10.5603/FHC.2012.0077

Bibliographic record

Folia Histochem Cytobiol 2012;50(4):547-553.

Authors

Adam Lukasiewicz
Joanna Reszec
Radoslaw Kowalewski
Lech Chyczewski
Urszula Lebkowska

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