open access

Vol 50, No 4 (2012)
Original paper
Submitted: 2012-11-24
Accepted: 2012-11-29
Published online: 2012-12-23
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Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Kamila Siedlecka-Kroplewska, Agnieszka Jozwik, Lucyna Kaszubowska, Anna Kowalczyk, Wojciech Boguslawski
DOI: 10.5603/FHC.2012.0080
·
Folia Histochem Cytobiol 2012;50(4):574-580.

open access

Vol 50, No 4 (2012)
ORIGINAL PAPERS
Submitted: 2012-11-24
Accepted: 2012-11-29
Published online: 2012-12-23

Abstract

Pterostilbene, a polyphenolic compound present in grapes and other fruits, has been demonstrated to inhibit growth and induce apoptosis and autophagy in some cancer cell types. We found that pterostilbene at the IC90 concentration of 44 µM inhibited proliferation and induced apoptosis in MOLT4 human leukemia cells. Treatment with pterostilbene resulted in a transient accumulation of cells in the G0/G1-cell cycle phase followed by the S-phase arrest. Pterostilbene-induced apoptotic death of MOLT4 cells was mediated by caspase-3 activation and was accompanied by the disruption of mitochondrial membrane potential, phosphatidylserine externalization and internucleosomal DNA fragmentation. Our results suggest that pterostilbene could serve as a potential additional chemotherapeutic agent for the treatment of leukemia.

Abstract

Pterostilbene, a polyphenolic compound present in grapes and other fruits, has been demonstrated to inhibit growth and induce apoptosis and autophagy in some cancer cell types. We found that pterostilbene at the IC90 concentration of 44 µM inhibited proliferation and induced apoptosis in MOLT4 human leukemia cells. Treatment with pterostilbene resulted in a transient accumulation of cells in the G0/G1-cell cycle phase followed by the S-phase arrest. Pterostilbene-induced apoptotic death of MOLT4 cells was mediated by caspase-3 activation and was accompanied by the disruption of mitochondrial membrane potential, phosphatidylserine externalization and internucleosomal DNA fragmentation. Our results suggest that pterostilbene could serve as a potential additional chemotherapeutic agent for the treatment of leukemia.
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Keywords

cell biology

About this article
Title

Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 4 (2012)

Article type

Original paper

Pages

574-580

Published online

2012-12-23

DOI

10.5603/FHC.2012.0080

Bibliographic record

Folia Histochem Cytobiol 2012;50(4):574-580.

Keywords

cell biology

Authors

Kamila Siedlecka-Kroplewska
Agnieszka Jozwik
Lucyna Kaszubowska
Anna Kowalczyk
Wojciech Boguslawski

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