open access

Vol 50, No 3 (2012)
Original paper
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08
Get Citation

DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules

Maria Kwiatkowska, Dariusz Stępiński, Katarzyna Popłońska, Agnieszka Wojtczak, Justyna T. Polit
DOI: 10.5603/FHC.2012.0058
·
Folia Histochem Cytobiol 2012;50(3):427-431.

open access

Vol 50, No 3 (2012)
ORIGINAL PAPERS
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08

Abstract

The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in
A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well
as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence
of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the
lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.
Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated
in lipid synthesis. A similar phenomenon may take place in A. thaliana.

Abstract

The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in
A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well
as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence
of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the
lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.
Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated
in lipid synthesis. A similar phenomenon may take place in A. thaliana.
Get Citation
About this article
Title

DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 3 (2012)

Article type

Original paper

Pages

427-431

Published online

2012-10-08

DOI

10.5603/FHC.2012.0058

Bibliographic record

Folia Histochem Cytobiol 2012;50(3):427-431.

Authors

Maria Kwiatkowska
Dariusz Stępiński
Katarzyna Popłońska
Agnieszka Wojtczak
Justyna T. Polit

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