open access

Vol 50, No 3 (2012)
Original paper
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08
Get Citation

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells

María Del Socorro Pina-Canseco, Araceli Páez-Arenas, Felipe Massó, Eduardo Pérez-Campos, Ruth Martínez-Cruz, Pedro Hernández-Cruz, Abraham Majluf-Cruz, Margarito Martínez-Cruz, Laura Pérez-Campos Mayoral, Alma Dolores Pérez-Santiago, Edgar Zenteno
DOI: 10.5603/FHC.2012.0055
·
Folia Histochem Cytobiol 2012;50(3):407-413.

open access

Vol 50, No 3 (2012)
ORIGINAL PAPERS
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08

Abstract

Activated protein C (APC) is generated from the cleavage of protein C by thrombin coupled to thrombomodulin
and, subsequently, is released as protein C activation peptide (papC). The aim of this study was to
evaluate the effect of papC on human dermal microvascular endothelial cells (HMEC-1), activated with 5 ng/
/mL TNF-a. Flow cytometry showed that papC inhibited the expression of VCAM-1 and ICAM-1, after activation
with TNF-a. Similarly, RT-PCR analysis revealed that 2 and 4 pM papC inhibited the expression of VCAM-1
and IL-8 mRNA in TNF-a-treated HMEC-1. In addition, the expression of endothelial nitric oxide synthase
(eNOS) increased in HMEC-1 treated with papC, compared to those without treatment. Furthermore, Jurkat
cell adhesion to HMEC-1 induced by TNF-a was significantly inhibited after the addition of papC, compared to
HMEC-1 without papC (p = 0.03). Finally, a control peptide analog to papC showed no effect on the expression
of ICAM and VCAM on the surface of HMEC-1. In conclusion, our results suggest that papC exerts antiinflammatory
effects on endothelial cells.

Abstract

Activated protein C (APC) is generated from the cleavage of protein C by thrombin coupled to thrombomodulin
and, subsequently, is released as protein C activation peptide (papC). The aim of this study was to
evaluate the effect of papC on human dermal microvascular endothelial cells (HMEC-1), activated with 5 ng/
/mL TNF-a. Flow cytometry showed that papC inhibited the expression of VCAM-1 and ICAM-1, after activation
with TNF-a. Similarly, RT-PCR analysis revealed that 2 and 4 pM papC inhibited the expression of VCAM-1
and IL-8 mRNA in TNF-a-treated HMEC-1. In addition, the expression of endothelial nitric oxide synthase
(eNOS) increased in HMEC-1 treated with papC, compared to those without treatment. Furthermore, Jurkat
cell adhesion to HMEC-1 induced by TNF-a was significantly inhibited after the addition of papC, compared to
HMEC-1 without papC (p = 0.03). Finally, a control peptide analog to papC showed no effect on the expression
of ICAM and VCAM on the surface of HMEC-1. In conclusion, our results suggest that papC exerts antiinflammatory
effects on endothelial cells.
Get Citation
About this article
Title

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 3 (2012)

Article type

Original paper

Pages

407-413

Published online

2012-10-08

DOI

10.5603/FHC.2012.0055

Bibliographic record

Folia Histochem Cytobiol 2012;50(3):407-413.

Authors

María Del Socorro Pina-Canseco
Araceli Páez-Arenas
Felipe Massó
Eduardo Pérez-Campos
Ruth Martínez-Cruz
Pedro Hernández-Cruz
Abraham Majluf-Cruz
Margarito Martínez-Cruz
Laura Pérez-Campos Mayoral
Alma Dolores Pérez-Santiago
Edgar Zenteno

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