open access

Vol 50, No 3 (2012)
Original paper
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08
Get Citation

Cell death in HeLa cells upon imperatorin and cisplatin treatment

Joanna Jakubowicz-Gil, Roman Paduch, Zofia Ulz, Dorota Badziul, Kazimierz Głowniak, Antoni Gawron
DOI: 10.5603/FHC.2012.0052
·
Folia Histochem Cytobiol 2012;50(3):381-391.

open access

Vol 50, No 3 (2012)
ORIGINAL PAPERS
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08

Abstract

There is growing evidence that commonly applied chemotherapy regimens can be improved by introducing
new, specific, active and low side-effect drugs, or by combining substances to obtain the required clinical
effect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separately
or in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line
(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. In
contrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resulted
only in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,
although to a smaller extent than that observed after imperatorin administered alone. At the molecular
level, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 light
chain LC3 — LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.
Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducer
in HeLa cells.

Abstract

There is growing evidence that commonly applied chemotherapy regimens can be improved by introducing
new, specific, active and low side-effect drugs, or by combining substances to obtain the required clinical
effect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separately
or in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line
(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. In
contrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resulted
only in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,
although to a smaller extent than that observed after imperatorin administered alone. At the molecular
level, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 light
chain LC3 — LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.
Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducer
in HeLa cells.
Get Citation
About this article
Title

Cell death in HeLa cells upon imperatorin and cisplatin treatment

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 3 (2012)

Article type

Original paper

Pages

381-391

Published online

2012-10-08

DOI

10.5603/FHC.2012.0052

Bibliographic record

Folia Histochem Cytobiol 2012;50(3):381-391.

Authors

Joanna Jakubowicz-Gil
Roman Paduch
Zofia Ulz
Dorota Badziul
Kazimierz Głowniak
Antoni Gawron

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl