open access

Vol 52, No 1 (2014)
Original paper
Submitted: 2012-07-24
Accepted: 2014-04-09
Published online: 2014-05-07
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A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

Murat Dereli, Bartlomiej E. Krazinski, Suleyman Ayvaz, Burhan Aksu, Mehmet Kanter, Hafize Uzun, Remise Gelisgen, C. Hasan Umit, Mustafa Inan, Umit Nusret Basaran, Mehmet Pul
DOI: 10.5603/FHC.2014.0003
·
Folia Histochem Cytobiol 2014;52(1):29-35.

open access

Vol 52, No 1 (2014)
ORIGINAL PAPERS
Submitted: 2012-07-24
Accepted: 2014-04-09
Published online: 2014-05-07

Abstract

Accidentally ingested corrosive substances can cause functional and structural damage to the esophageal tissue resulting in stricture formation. It has been reported that the administration of olmesartan (OLM) can have anti-inflammatory, antifibrotic and antiapoptotic effects on injured tissue. The aim of our study was to check if OLM could prevent formation of scars in the corrosive esophageal burn model. Fifty-one Wistar Albino rats were divided into six groups: Control, Sham, OLM, Sham + OLM, Burn, and Burn + OLM. Olmesartan (5 mg/kg) was given by gavage once per day for 21 consecutive days after injury. The morphology of the esophagus was assessed after Masson trichrome staining, and apoptosis was evaluated using the terminal deoxynucleotidyl transferased UTP nick end labeling (TUNEL) method. The serum nucleosomes (as an indicator of apoptosis), serum p53 protein, and esophageal tissue p53 protein levels of each group were measured by immunoassays. Muscularis mucosa damage, submucosal collagen deposition, and tunica muscularis injury in the Burn + OLM group decreased significantly compared with the Burn group (p < 0.05). Similarly, the number of apoptotic cells in the Burn + OLM group decreased compared with the Burn group (p < 0.05). Serum levels of nucleosomes and p53 and tissue of p53 protein did not differ between the groups. Exogenously administered OLM can effectively prevent the occurrence of esophageal strictures caused by corrosive esophageal burns. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 29–35)

Abstract

Accidentally ingested corrosive substances can cause functional and structural damage to the esophageal tissue resulting in stricture formation. It has been reported that the administration of olmesartan (OLM) can have anti-inflammatory, antifibrotic and antiapoptotic effects on injured tissue. The aim of our study was to check if OLM could prevent formation of scars in the corrosive esophageal burn model. Fifty-one Wistar Albino rats were divided into six groups: Control, Sham, OLM, Sham + OLM, Burn, and Burn + OLM. Olmesartan (5 mg/kg) was given by gavage once per day for 21 consecutive days after injury. The morphology of the esophagus was assessed after Masson trichrome staining, and apoptosis was evaluated using the terminal deoxynucleotidyl transferased UTP nick end labeling (TUNEL) method. The serum nucleosomes (as an indicator of apoptosis), serum p53 protein, and esophageal tissue p53 protein levels of each group were measured by immunoassays. Muscularis mucosa damage, submucosal collagen deposition, and tunica muscularis injury in the Burn + OLM group decreased significantly compared with the Burn group (p < 0.05). Similarly, the number of apoptotic cells in the Burn + OLM group decreased compared with the Burn group (p < 0.05). Serum levels of nucleosomes and p53 and tissue of p53 protein did not differ between the groups. Exogenously administered OLM can effectively prevent the occurrence of esophageal strictures caused by corrosive esophageal burns. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 29–35)

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Keywords

corrosive esophageal burn; stricture; olmesartan; protection; apoptosis; TUNEL; nucleosomes; p53

About this article
Title

A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 1 (2014)

Article type

Original paper

Pages

29-35

Published online

2014-05-07

DOI

10.5603/FHC.2014.0003

Bibliographic record

Folia Histochem Cytobiol 2014;52(1):29-35.

Keywords

corrosive esophageal burn
stricture
olmesartan
protection
apoptosis
TUNEL
nucleosomes
p53

Authors

Murat Dereli
Bartlomiej E. Krazinski
Suleyman Ayvaz
Burhan Aksu
Mehmet Kanter
Hafize Uzun
Remise Gelisgen
C. Hasan Umit
Mustafa Inan
Umit Nusret Basaran
Mehmet Pul

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