open access

Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Accepted: 2012-04-24
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Surface Plasmon Resonance Imaging biosensor for cystatin determination based on the application of bromelain, ficin and chymopapain

Ewa Gorodkiewicz, Joanna Breczko, Anna Sankiewicz
DOI: 10.5603/FHC.2012.0019
·
Folia Histochem Cytobiol 2012;50(1):130-136.

open access

Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Accepted: 2012-04-24

Abstract

A Surface Plasmon Resonance Imaging (SPRI) sensor based on bromelain or chymopapain or ficin has been developed for specific cystatin determination. Cystatin was captured from a solution by immobilized bromelain or chymopapain or ficin due to the formation of an enzyme-inhibitor complex on the biosensor surface. The influence of bromelain, chymopapain or ficin concentration, as well as the pH of the interaction on the SPRI signal, was investigated and optimized. Sensor dynamic response range is between 0–0.6 μg/ml and the detection limit is equal to 0.1 μg/ml. In order to demonstrate the sensor potential, cystatin was determined in blood plasma, urine and saliva, showing good agreement with the data reported in the literature.

Abstract

A Surface Plasmon Resonance Imaging (SPRI) sensor based on bromelain or chymopapain or ficin has been developed for specific cystatin determination. Cystatin was captured from a solution by immobilized bromelain or chymopapain or ficin due to the formation of an enzyme-inhibitor complex on the biosensor surface. The influence of bromelain, chymopapain or ficin concentration, as well as the pH of the interaction on the SPRI signal, was investigated and optimized. Sensor dynamic response range is between 0–0.6 μg/ml and the detection limit is equal to 0.1 μg/ml. In order to demonstrate the sensor potential, cystatin was determined in blood plasma, urine and saliva, showing good agreement with the data reported in the literature.
Get Citation

Keywords

SPRI biosensor; cystatins; bromelain; ficin; chymopapain

About this article
Title

Surface Plasmon Resonance Imaging biosensor for cystatin determination based on the application of bromelain, ficin and chymopapain

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 1 (2012)

Pages

130-136

DOI

10.5603/FHC.2012.0019

Bibliographic record

Folia Histochem Cytobiol 2012;50(1):130-136.

Keywords

SPRI biosensor
cystatins
bromelain
ficin
chymopapain

Authors

Ewa Gorodkiewicz
Joanna Breczko
Anna Sankiewicz

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