open access

Vol 50, No 1 (2012)
Original paper
Submitted: 2012-04-24
Accepted: 2012-04-24
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Increased apoptotic activity on inflammatory human placentas in spontaneous abortions during the first and second trimester of gestation: a histochemical and immunohistochemical study

Theodora-Eleftheria Deftereou, Maria Lambropoulou, Ekaterini Chatzaki, Maria Koffa, Petros Ypsilantis, Olga Pagonopoulou, Iordana Grammatikopoulou, Elias Papadopoulos, Alexandros Papalambros, Emmanouil Kontomanolis, Nikolas Papadopoulos
DOI: 10.5603/FHC.2012.0017
·
Folia Histochem Cytobiol 2012;50(1):118-124.

open access

Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Accepted: 2012-04-24

Abstract

The aim of this study was to investigate the role of apoptotic markers on inflammatory human placentas from spontaneous abortions during the first and second trimester of gestation and compare them to those without inflammation. Paraffin-embedded specimens from 76 placentas were investigated by conventional histology and immunohistochemistry using monoclonal antibodies against M30, Caspase 3, Caspase 8 and Caspase 9, as well as the terminal deoxynucleotidyl tranferase-mediated deoxyuridine triphosphate nick end labeling method. A higher prevalence of expression of apoptotic markers (94.4%) was observed in placentas associated with chorioamnionitis in comparison with those without inflammation. Our observations confirm that apoptosis is strikingly prevalent in placentas diagnosed with histologic chorioamnionitis, while the inflammation induces cell death.

Abstract

The aim of this study was to investigate the role of apoptotic markers on inflammatory human placentas from spontaneous abortions during the first and second trimester of gestation and compare them to those without inflammation. Paraffin-embedded specimens from 76 placentas were investigated by conventional histology and immunohistochemistry using monoclonal antibodies against M30, Caspase 3, Caspase 8 and Caspase 9, as well as the terminal deoxynucleotidyl tranferase-mediated deoxyuridine triphosphate nick end labeling method. A higher prevalence of expression of apoptotic markers (94.4%) was observed in placentas associated with chorioamnionitis in comparison with those without inflammation. Our observations confirm that apoptosis is strikingly prevalent in placentas diagnosed with histologic chorioamnionitis, while the inflammation induces cell death.
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Keywords

placenta; apoptosis; Immunohistochemistry; congenital disorders; chorioamnionitis

About this article
Title

Increased apoptotic activity on inflammatory human placentas in spontaneous abortions during the first and second trimester of gestation: a histochemical and immunohistochemical study

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 1 (2012)

Article type

Original paper

Pages

118-124

DOI

10.5603/FHC.2012.0017

Bibliographic record

Folia Histochem Cytobiol 2012;50(1):118-124.

Keywords

placenta
apoptosis
Immunohistochemistry
congenital disorders
chorioamnionitis

Authors

Theodora-Eleftheria Deftereou
Maria Lambropoulou
Ekaterini Chatzaki
Maria Koffa
Petros Ypsilantis
Olga Pagonopoulou
Iordana Grammatikopoulou
Elias Papadopoulos
Alexandros Papalambros
Emmanouil Kontomanolis
Nikolas Papadopoulos

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