open access

Vol 50, No 3 (2012)
ORIGINAL PAPERS
Published online: 2012-10-08
Submitted: 2012-01-24
Accepted: 2012-06-06
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Structure of the nucleoli in domestic cattle spermatocytes

Katarzyna Andraszek, Elżbieta Smalec
DOI: 10.5603/FHC.2012.0047
·
Folia Histochem Cytobiol 2012;50(3):346-351.

open access

Vol 50, No 3 (2012)
ORIGINAL PAPERS
Published online: 2012-10-08
Submitted: 2012-01-24
Accepted: 2012-06-06

Abstract

The work was aimed at determining the number and morphology of nucleoli in the prophase of the first meiotic division in domestic cattle males. The use of AgNO3 staining, commonly applied in cytogenetics for the identification of nucleolar organiser regions, made it possible to identify nucleoli in first-order spermatocytes. One nucleolus was identified in each analysed cell. Considerable morphological differentiation of the nucleoli during the prophase of the first meiotic division, particularly in leptotene, unobserved in other farm animal species, was noticed. Dark-hued grain-like structures were found within the disintegrating nucleoli, corresponding approximately or exactly to the number of the nucleolar organiser regions in the domestic cattle karyotype. Dark areas were identified in the selected prometaphase chromosomes. Their number corresponded with the number of active NORs defined in the domestic cattle karyotype.

Abstract

The work was aimed at determining the number and morphology of nucleoli in the prophase of the first meiotic division in domestic cattle males. The use of AgNO3 staining, commonly applied in cytogenetics for the identification of nucleolar organiser regions, made it possible to identify nucleoli in first-order spermatocytes. One nucleolus was identified in each analysed cell. Considerable morphological differentiation of the nucleoli during the prophase of the first meiotic division, particularly in leptotene, unobserved in other farm animal species, was noticed. Dark-hued grain-like structures were found within the disintegrating nucleoli, corresponding approximately or exactly to the number of the nucleolar organiser regions in the domestic cattle karyotype. Dark areas were identified in the selected prometaphase chromosomes. Their number corresponded with the number of active NORs defined in the domestic cattle karyotype.

Get Citation
About this article
Title

Structure of the nucleoli in domestic cattle spermatocytes

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 3 (2012)

Pages

346-351

Published online

2012-10-08

DOI

10.5603/FHC.2012.0047

Bibliographic record

Folia Histochem Cytobiol 2012;50(3):346-351.

Authors

Katarzyna Andraszek
Elżbieta Smalec

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