Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)

Kamil Torres, Hanna Trębacz, Andrzej Chrościcki, Łukasz Pietrzyk, Anna Torres
DOI: 10.5603/FHC.2011.0094
Folia Histochem Cytobiol 2011;49(4):700-705.

open access

Vol 49, No 4 (2011)
Original Papers
Published online: 2012-01-16
Submitted: 2012-01-16


Abdominal surgeries alter the integrity of the peritoneal layer and cause imbalances among immunological, inflammatory and angiogenic mechanisms within the tissue. During laparoscopic procedures a protective function of the peritoneal layer can be disturbed by the gas used to create a pneumoperitoneum. The aim of this study was to characterize peritoneal tissue by means of differential scanning calorimetry (DSC) as a reference for future investigations on the influence of surgical procedures on the physicochemical state of the peritoneum. Thirty-seven patients participated in the study. Patients were divided into three groups according to the type of surgery: group H — patients who underwent hernia repair; group Ch — patients who underwent laparoscopic cholecystectomy; and group C — patients operated due to rectal cancer. It was observed that onset temperature (To), denaturation temperature (Tm) and change of enthalpy (ΔH) during thermal denaturation of peritoneal collagen in were significantly different for these three groups of patients. The mean values of onset temperature (To) and denaturation temperature (Tm) in group H were significantly lower, while DH in this group was significantly higher than in the two other groups (Ch and C). This preliminary study does not answer whether the differences in collagen denaturation found in peritoneal tissue from different groups of patients resulted from a different inherent state of the tissue, or from surgical procedures. However, the results suggest that DSC is an appropriate method to study subtle changes in the physicochemical condition of the peritoneum using small samples obtained during surgical procedures. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 700–705)


differential scanning calorimetry; peritoneum; pneumoperitoneum; collagen; laparoscopy

About this article

Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)


Folia Histochemica et Cytobiologica


Vol 49, No 4 (2011)



Published online




Bibliographic record

Folia Histochem Cytobiol 2011;49(4):700-705.


differential scanning calorimetry


Kamil Torres
Hanna Trębacz
Andrzej Chrościcki
Łukasz Pietrzyk
Anna Torres

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl